fiber (AC_000019) Virus Tagged ORF Clone

CAT#: VC100103

Myc-DDK-tagged ORF clone of viral ORF for fiber [Human adenovirus 35], codon optimized for human cell expression, AP_000601

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: fiber
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100103 represents NCBI reference of AP_000601 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTAAGAGAGTGAGATTGTCTGACTCTTTTAATCCTGTTTATCCTTACGAAGATGAGTCTACCAGTC
  AACACCCCTTCATCAATCCCGGATTCATTTCACCAAACGGATTTACCCAGAGCCCCGACGGGGTCCTCAC
  ACTCAAATGCCTGACACCACTGACAACAACGGGAGGCTCTTTGCAGCTCAAAGTTGGCGGGGGCTTGACA
  GTCGACGATACAGATGGGACCCTGCAAGAAAACATCCGGGCAACGGCTCCCATCACGAAAAACAATCATA
  GCGTAGAGCTGAGCATCGGTAACGGTCTGGAAACCCAGAACAACAAACTGTGTGCCAAACTGGGGAACGG
  CCTGAAGTTCAACAATGGTGACATCTGTATTAAGGATAGCATAAACACACTCTGGACTGGAATCAATCCC
  CCCCCGAACTGCCAGATCGTGGAGAACACCAATACTAACGATGGAAAGCTGACCTTGGTGCTTGTGAAGA
  ATGGCGGTCTTGTTAACGGGTACGTGAGCCTTGTGGGCGTGTCCGATACTGTTAACCAGATGTTTACACA
  AAAAACCGCTAATATCCAGCTGCGCCTGTACTTCGACAGCTCCGGGAATCTCCTTACCGAAGAGTCTGAT
  CTGAAAATCCCGCTGAAAAACAAGTCAAGCACAGCCACTAGTGAAACTGTTGCGTCTAGTAAGGCGTTTA
  TGCCAAGTACCACTGCCTACCCATTTAACACAACGACCCGGGACTCTGAAAACTACATCCACGGCATATG
  TTATTATATGACAAGCTACGATCGGTCCCTTTTCCCTCTCAATATTAGCATTATGTTGAACTCAAGGATG
  ATATCTTCTAACGTGGCCTACGCCATCCAGTTCGAATGGAACTTGAACGCTTCCGAAAGTCCAGAGAGCA
  ATATCGCGACATTGACCACATCTCCCTTTTTTTTCTCTTACATTACCGAGGACGATAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100103 representing AP_000601
  Red=Cloning sites Green=Tags
  
  MTKRVRLSDSFNPVYPYEDESTSQHPFINPGFISPNGFTQSPDGVLTLKCLTPLTTTGGSLQLKVGGGLT
  VDDTDGTLQENIRATAPITKNNHSVELSIGNGLETQNNKLCAKLGNGLKFNNGDICIKDSINTLWTGINP
  PPNCQIVENTNTNDGKLTLVLVKNGGLVNGYVSLVGVSDTVNQMFTQKTANIQLRLYFDSSGNLLTEESD
  LKIPLKNKSSTATSETVASSKAFMPSTTAYPFNTTTRDSENYIHGICYYMTSYDRSLFPLNISIMLNSRM
  ISSNVAYAIQFEWNLNASESPESNIATLTTSPFFFSYITEDDN
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000019
• ORF Size: 969 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000019.1, AP_000601
• RefSeq ORF: 969
• MW: 35.3 kDa