IX (AC_000019) Virus Tagged ORF Clone

CAT#: VC100076

Myc-DDK-tagged ORF clone of viral ORF for IX [Human adenovirus 35], codon optimized for human cell expression, AP_000574

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: IX
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100076 represents NCBI reference of AP_000574 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGGTAATGCGTCCTTTAAGGGAGGGGTGTTCAGCCCTTACCTGACTGGCCGGCTGCCTAGCTGGG
  CGGGGGTACGACAGAACGTCATGGGCAGTACTGTGGACGGCAGACCTGTGCAGCCTGCGAACTCCAGCAC
  ACTTACATACGCAACTTTGTCAAGCAGCCCCCTGGATGCCGCAGCCGCCGCTGCGGCAGCCAGCGTGGCT
  GCAAACACGGTGCTGGGTATGGGTTACTATGGATCAATCGTCGCTAATTCCACATCATCTAATAACCCCA
  GTACCCTGACACAAGATAAGCTTCTGGTACTGTTGGCCCAACTGGAGGCCCTGACACAACGGCTCGGAGA
  ACTCTCACAGCAGGTGGCCGAACTGAGGGTGCAGACGGAGTCTGCTGTTGGGACGGCGAAGAGTAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100076 representing AP_000574
  Red=Cloning sites Green=Tags
  
  MSGNASFKGGVFSPYLTGRLPSWAGVRQNVMGSTVDGRPVQPANSSTLTYATLSSSPLDAAAAAAAASVA
  ANTVLGMGYYGSIVANSTSSNNPSTLTQDKLLVLLAQLEALTQRLGELSQQVAELRVQTESAVGTAKSK
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000019
• ORF Size: 417 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000019.1, AP_000574
• RefSeq ORF: 417
• MW: 14.2 kDa