hexon (AC_000019) Virus Tagged ORF Clone

CAT#: VC100087

Myc-DDK-tagged ORF clone of viral ORF for hexon [Human adenovirus 35], codon optimized for human cell expression, AP_000585

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: hexon
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100087 represents NCBI reference of AP_000585 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACACCTTCCATGCTCCCCCAATGGGCCTACATGCATATCGCAGGACAGGACGCTAGTGAATATC
  TGTCTCCAGGTCTGGTGCAGTTTGCCAGAGCCACGGATACCTACTTCAACCTTGGAAACAAGTTCAGGAA
  TCCTACCGTGGCCCCTACCCACGACGTCACCACAGACAGAAGCCAACGCCTTATGTTGAGATTCGTGCCT
  GTTGATCGGGAAGATAACACTTATTCTTACAAAGTCCGCTACACCCTCGCTGTGGGGGATAACCGAGTGC
  TTGATATGGCGAGTACCTTTTTCGATATTAGAGGAGTACTGGACAGAGGCCCCAGTTTTAAGCCGTACTC
  TGGGACAGCGTATAACTCTTTGGCTCCCAAGGGTGCTCCCAATGCCAGCCAGTGGATCGCAAAAGGAGTT
  CCAACCGCAGCCGCTGCCGGAAACGGTGAGGAGGAGCATGAAACCGAGGAGAAAACTGCAACATATACCT
  TCGCTAATGCCCCAGTTAAAGCCGAAGCTCAAATCACGAAGGAGGGCTTGCCCATCGGCTTGGAAATCAG
  TGCGGAGAACGAATCTAAGCCAATCTACGCAGATAAGCTGTACCAGCCCGAACCACAGGTGGGAGACGAA
  ACTTGGACGGACCTGGACGGAAAGACAGAGGAATACGGCGGTCGAGCCCTTAAGCCTACAACGAATATGA
  AACCCTGTTACGGAAGCTACGCTAAACCTACCAATCTCAAGGGCGGTCAGGCGAAACCTAAAAATTCCGA
  GCCAAGCAGCGAGAAGATTGAATACGACATCGATATGGAGTTCTTCGACAACTCTAGCCAACGCACAAAT
  TTTAGCCCCAAGATTGTCATGTACGCGGAAAATGTTGGGCTGGAGACTCCTGATACACACGTGGTGTACA
  AGCCTGGTACTGAGGACACCTCCTCCGAGGCGAACCTGGGCCAGCAAAGTATGCCGAATCGACCCAATTA
  CATCGGGTTTCGCGATAATTTCATAGGGCTGATGTACTACAATTCCACTGGCAACATGGGAGTGCTGGCC
  GGCCAGGCTAGCCAACTCAACGCCGTGGTAGATCTTCAAGACCGGAACACCGAGCTTAGCTATCAGTTGC
  TCCTCGACTCCCTTGGAGACCGGACCCGATACTTCTCAATGTGGAATCAGGCTGTCGACAGTTACGATCC
  TGATGTGCGGGTCATTGAAAACCATGGTGTGGAGGATGAACTTCCCAACTACTGCTTCCCACTGGATGGC
  ATCGGTGTTCCCACCACCAGCTACAAAAGCATCGTGCCGAACGGAGAAGATAACAACAACTGGAAGGAGC
  CGGAAGTCAACGGCACATCCGAAATTGGCCAGGGCAACCTCTTTGCTATGGAGATCAACCTGCAGGCCAA
  CCTTTGGCGGTCTTTTTTGTACAGTAATGTCGCCCTCTATCTTCCAGACTCTTACAAATATACCCCGAGC
  AACGTGACTCTTCCTGAGAATAAGAACACCTATGATTATATGAATGGTAGGGTCGTACCACCCTCCCTGG
  TAGACACATATGTGAATATCGGGGCCAGGTGGTCCCTGGATGCCATGGACAATGTGAACCCCTTTAATCA
  TCACCGAAACGCTGGGTTGCGCTACCGCTCTATGCTGTTGGGGAACGGACGCTACGTGCCTTTCCACATA
  CAGGTGCCACAAAAATTTTTCGCAGTGAAGAACCTTCTCCTGCTCCCTGGAAGCTATACCTACGAGTGGA
  ACTTCAGAAAGGATGTAAACATGGTGCTGCAGTCTTCTCTGGGCAACGATCTGAGAGTTGATGGAGCCAG
  CATCAGTTTCACTTCCATTAATCTCTACGCTACTTTCTTTCCCATGGCCCATAACACCGCAAGCACGCTT
  GAGGCGATGTTGCGCAATGATACAAACGATCAGAGTTTCAACGATTATTTGTCCGCTGCGAATATGCTGT
  ATCCGATTCCCGCCAACGCCACCAATATCCCCATCTCAATCCCTTCCCGGAATTGGGCCGCCTTTAGAGG
  CTGGTCTTTCACACGCCTGAAAACCAAGGAGACCCCATCTCTCGGATCTGGGTTTGACCCGTACTTCGTC
  TACAGTGGATCCATTCCCTACCTGGACGGGACATTCTACCTCAATCACACATTCAAAAAAGTGTCTATTA
  TGTTCGACAGCTCTGTGTCCTGGCCCGGCAATGACCGCCTGCTGAGTCCCAATGAGTTCGAGATAAAAAG
  AACCGTTGACGGGGAGGGCTACAACGTGGCCCAGTGCAATATGACCAAGGACTGGTTTCTCGTGCAGATG
  TTGGCTAACTATAACATTGGCTACCAAGGCTTCTACATTCCCGAGGGGTATAAGGATCGAATGTACTCCT
  TCTTTAGAAACTTTCAGCCGATGTCCAGGCAGGTGGTCGATGAAGTGAATTACAAGGATTTTAAGGCCGT
  TGCAATCCCATATCAGCACAATAATTCAGGATTCGTAGGGTACATGGCCCCAACCATGAGACAGGGCCAG
  CCGTATCCCGCAAATTACCCCTATCCCCTGATCGGTACGACCGCCGTGAACAGCGTTACACAGAAGAAGT
  TTCTGTGTGATCGGACTATGTGGAGGATACCCTTTTCCTCTAATTTTATGAGCATGGGGGCACTGACCGA
  CCTGGGCCAGAACATGCTGTACGCTAATAGCGCCCACGCTCTGGACATGACATTCGAGGTGGATCCTATG
  GACGAGCCAACCCTCCTCTACCTCCTTTTCGAGGTCTTTGATGTCGTCCGGGTCCACCAGCCTCACCGAG
  GTATTATCGAGGCTGTATACTTGCGGACTCCTTTCTCTGCCGGCAATGCTACTACA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100087 representing AP_000585
  Red=Cloning sites Green=Tags
  
  MATPSMLPQWAYMHIAGQDASEYLSPGLVQFARATDTYFNLGNKFRNPTVAPTHDVTTDRSQRLMLRFVP
  VDREDNTYSYKVRYTLAVGDNRVLDMASTFFDIRGVLDRGPSFKPYSGTAYNSLAPKGAPNASQWIAKGV
  PTAAAAGNGEEEHETEEKTATYTFANAPVKAEAQITKEGLPIGLEISAENESKPIYADKLYQPEPQVGDE
  TWTDLDGKTEEYGGRALKPTTNMKPCYGSYAKPTNLKGGQAKPKNSEPSSEKIEYDIDMEFFDNSSQRTN
  FSPKIVMYAENVGLETPDTHVVYKPGTEDTSSEANLGQQSMPNRPNYIGFRDNFIGLMYYNSTGNMGVLA
  GQASQLNAVVDLQDRNTELSYQLLLDSLGDRTRYFSMWNQAVDSYDPDVRVIENHGVEDELPNYCFPLDG
  IGVPTTSYKSIVPNGEDNNNWKEPEVNGTSEIGQGNLFAMEINLQANLWRSFLYSNVALYLPDSYKYTPS
  NVTLPENKNTYDYMNGRVVPPSLVDTYVNIGARWSLDAMDNVNPFNHHRNAGLRYRSMLLGNGRYVPFHI
  QVPQKFFAVKNLLLLPGSYTYEWNFRKDVNMVLQSSLGNDLRVDGASISFTSINLYATFFPMAHNTASTL
  EAMLRNDTNDQSFNDYLSAANMLYPIPANATNIPISIPSRNWAAFRGWSFTRLKTKETPSLGSGFDPYFV
  YSGSIPYLDGTFYLNHTFKKVSIMFDSSVSWPGNDRLLSPNEFEIKRTVDGEGYNVAQCNMTKDWFLVQM
  LANYNIGYQGFYIPEGYKDRMYSFFRNFQPMSRQVVDEVNYKDFKAVAIPYQHNNSGFVGYMAPTMRQGQ
  PYPANYPYPLIGTTAVNSVTQKKFLCDRTMWRIPFSSNFMSMGALTDLGQNMLYANSAHALDMTFEVDPM
  DEPTLLYLLFEVFDVVRVHQPHRGIIEAVYLRTPFSAGNATT
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000019
• ORF Size: 2856 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000019.1, AP_000585
• RefSeq ORF: 2856
• MW: 107.3 kDa