IVa2 (AC_000019) Virus Tagged ORF Clone

CAT#: VC100077

Myc-DDK-tagged ORF clone of viral ORF for IVa2 [Human adenovirus 35], codon optimized for human cell expression, AP_000575

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: IVa2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100077 represents NCBI reference of AP_000575 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAACAAAAGGCCGCCAGGCAACTGAAGTCCTCCACCAACAGAATGAGTCCGAAGCGCACCCTGGCA
  AGAGACCATCTCGACGGACCCCTCTCCATTGCGACGGCAATAATCCTGATTCAAACACTGAAACCCTGGA
  AGGCCCTAATCCGGGATCCGCCGGCCGGCCTTCAAGCCACACCCTGCTGCAGGAACCATCAAAACCCGCA
  AAGAGAGGTGGACTGCTCGACCGGGACGCTATTGAACACATCACGGAGCTTTGGAATCGACTGGAACTGT
  TGCAACAGACCCTGTCAAAAATGCCCATGGCCGACGGCCTTAAACCTCTGAAGAATTTTTCTTCTCTTCA
  GGAGCTGCTGAGCCTGGGAGGCGAGAGGCTGCTGATGGATCTGGTTAGGGAGAATATGCACGTCAGAGAG
  ATGATGAATGAAGTCGCTCCACTGCTGCGGGAAGACGGATCATGTCGGAGCCTGAATTACCATCTGCAGC
  CAGTAATCGGCATCATCTATGGACCCACCGGGTGCGGCAAGTCTCAATTGCTCCGAAATTTGCTCTCTAG
  CCAGCTCATCACTCCTGCTCCAGAAACAGTGTTTTTCATCGCCCCACAGGTGGACATGATTCCGCCATCC
  GAGCTTAAAGCCTGGGAAATGCAAATTTGCGAGGGTAACTACGCTCCGGGGCCTGAGGGAACATTCATAC
  CCCAGTCCGGTACCCTTCGGCCAAAATTCATAAAGATGGCCTACGACGACTTGACACAGGAACACAATTA
  CGACGTGTCTGACCCAAGAAACGTTTTCGCACGCGCAGCGGCACATGGCCCTATCGCTATCATAATGGAC
  GAATGCATGGAGAACCTGGGGGGGCACAAAGGTGTCTCCAAGTTCTTTCATGCTTTCCCCAGCAAACTGC
  ACGACAAGTTTCCAAAGTGCACTGGGTACACTGTGCTCGTAGTTTTGCACAACATGAATCCTCGCCGGGA
  CCTCGGCGGCAACATAGCTAACCTGAAAATCCAGTCAAAAATGCACATCATCTCACCTAGAATGCATCCT
  TCCCAGTTGAATAGGTTTGTTAACACCTACACAAAAGGCCTCTCCGTGGCCATCTCCTTGCTCCTGAAGG
  ACATCGTGCAGCACCATGCTCTCAGACCCTGCTATGATTGGGTGATCTATAACACCACTCCTGAGCACGA
  GTCCCTGCAGTGGAGTTACCTGCATCCCAAGGACGGGTTGATGCCCATGTACCTTAATATCCAGTCTCAT
  CTGTATCGAATCCTGGAAAAGATCCATCGGGTGCTCAATGACAGGGATAGGTGGAGTAGAGCATACAGAG
  CACGCAAAATGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100077 representing AP_000575
  Red=Cloning sites Green=Tags
  
  METKGRQATEVLHQQNESEAHPGKRPSRRTPLHCDGNNPDSNTETLEGPNPGSAGRPSSHTLLQEPSKPA
  KRGGLLDRDAIEHITELWNRLELLQQTLSKMPMADGLKPLKNFSSLQELLSLGGERLLMDLVRENMHVRE
  MMNEVAPLLREDGSCRSLNYHLQPVIGIIYGPTGCGKSQLLRNLLSSQLITPAPETVFFIAPQVDMIPPS
  ELKAWEMQICEGNYAPGPEGTFIPQSGTLRPKFIKMAYDDLTQEHNYDVSDPRNVFARAAAHGPIAIIMD
  ECMENLGGHKGVSKFFHAFPSKLHDKFPKCTGYTVLVVLHNMNPRRDLGGNIANLKIQSKMHIISPRMHP
  SQLNRFVNTYTKGLSVAISLLLKDIVQHHALRPCYDWVIYNTTPEHESLQWSYLHPKDGLMPMYLNIQSH
  LYRILEKIHRVLNDRDRWSRAYRARKMK
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000019
• ORF Size: 1344 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000019.1, AP_000575
• RefSeq ORF: 1344
• MW: 50.8 kDa