protease (AC_000019) Virus Tagged ORF Clone

CAT#: VC100088

Myc-DDK-tagged ORF clone of viral ORF for protease [Human adenovirus 35], codon optimized for human cell expression, AP_000586

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: protease
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100088 represents NCBI reference of AP_000586 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTGTGGTTCCCAGAACGGGTCATCCGAGCAGGAACTCAGGGCAATTGTGCAGGACTTGGGCTGCG
  GGCCATACTTCCTCGGTACATATGATAAAAGATTTCCTGGCTTTATGGCCCCCGATAAGTTGGCGTGTGC
  GATAGTTAACACGGCTGGACGCGAAACGGGCGGGGAGCATTGGCTGGCTTTTGGGTGGAACCCTCGGAGT
  AACACTTGCTACCTCTTTGATCCTTTCGGCTTCAGCGATGATAGGTTGAAGCAAATCTATCAGTTTGAGT
  ATGAAGGCCTCCTGAGACGCTCTGCCCTCGCTACAAAAGATCGCTGTATCACCCTGGAGAAAAGTACCCA
  GACCGTGCAAGGTCCCCGATCAGCCGCATGCGGATTGTTCTGTTGCATGTTCCTCCATGCCTTCGTGCAC
  TGGCCTGACAGGCCCATGGATGGCAACCCAACCATGAAGTTGCTGACCGGCGTGCCAAACAATATGTTGC
  ATTCTCCCAAGGTGCAGCCTACCTTGTGTGATAACCAGAAGGCGCTGTATCACTTCCTCAATACTCACAG
  TCCTTACTTCCGGTCCCACAGAACTCACATCGAGAGGGCGACCGCATTTGACAGAATGGACGTGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100088 representing AP_000586
  Red=Cloning sites Green=Tags
  
  MACGSQNGSSEQELRAIVQDLGCGPYFLGTYDKRFPGFMAPDKLACAIVNTAGRETGGEHWLAFGWNPRS
  NTCYLFDPFGFSDDRLKQIYQFEYEGLLRRSALATKDRCITLEKSTQTVQGPRSAACGLFCCMFLHAFVH
  WPDRPMDGNPTMKLLTGVPNNMLHSPKVQPTLCDNQKALYHFLNTHSPYFRSHRTHIERATAFDRMDVQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000019
• ORF Size: 627 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000019.1, AP_000586
• RefSeq ORF: 627
• MW: 23.7 kDa