E1B 19K (AC_000019) Virus Tagged ORF Clone

CAT#: VC100074

Myc-DDK-tagged ORF clone of viral ORF for E1B 19K [Human adenovirus 35], codon optimized for human cell expression, AP_000572

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E1B 19K
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100074 represents NCBI reference of AP_000572 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGTGTGGGCGATCCTGGAGGATTTGAGGAAGACACGCCAGCTGCTGGAGAGTGCAAGCGACGGGG
  TTAGCGGATTCTGGCGGTTCTGGTTTGCCAGCGAACTGGCACGGGTTGTCTTTCGAATAAAGCAGGACTA
  CAAACAGGAGTTCGAGAAACTGCTGGTCGATTGCCCAGGACTGTTCGAGGCTCTGAACCTGGGACACCAA
  GTTCATTTTAAAGAAAAGGTACTGAGTGTCCTCGATTTCAGCACCCCAGGGAGGACTGCCGCCGCCGTCG
  CCTTTCTGACATTCATCCTTGATAAGTGGATCCCTCAAACTCACTTTTCTAGGGGTTATGTGCTGGATTT
  TATCGCGACAGCTCTCTGGAGGACTTGGAAGGTGCGCAAGATGCGCACTATTCTGGGGTATTGGCCCGTC
  CAGCCACTTGGCGTGGCCGGAATACTGAGACACCCTCCCGTAATGCCTGCCGTCCTCGAAGAGGAGCAAC
  AAGAAGACAATCCCAGAGCAGGACTCGATCCTCCCGTCGAGGAGGCTGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100074 representing AP_000572
  Red=Cloning sites Green=Tags
  
  MEVWAILEDLRKTRQLLESASDGVSGFWRFWFASELARVVFRIKQDYKQEFEKLLVDCPGLFEALNLGHQ
  VHFKEKVLSVLDFSTPGRTAAAVAFLTFILDKWIPQTHFSRGYVLDFIATALWRTWKVRKMRTILGYWPV
  QPLGVAGILRHPPVMPAVLEEEQQEDNPRAGLDPPVEEAE
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000019
• ORF Size: 540 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000019.1, AP_000572
• RefSeq ORF: 540
• MW: 20.8 kDa