E4 ORFB (AC_000008) Virus Tagged ORF Clone

CAT#: VC100144

Myc-DDK-tagged ORF clone of viral ORF for E4 ORFB [Human adenovirus 5], codon optimized for human cell expression, AP_000231

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E4 ORFB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100144 represents NCBI reference of AP_000231 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCGAGAGAAAAATGGTTAGTTTTAGTGTGGTCGTGCCGGAACTTACCTGTCTCTACTTGCACGAAC
  ACGATTACGACGTGTTGTCCTTCCTGAGAGAGGCCTTGCCAGACTTTTTGAGCAGTACCCTTCACTTTAT
  ATCTCCTCCTATGCAACAAGCCTATATAGGTGCAACATTGGTTAGTATAGCTCCTAGTATGAGGGTGATC
  ATCAGCGTGGGTTCCTTTGTGATGGTCCCTGGGGGTGAAGTGGCAGCCCTCGTAAGAGCCGATCTGCACG
  ACTACGTCCAACTGGCCCTTAGGAGAGACCTCAGGGATCGAGGAATCTTCGTGAACGTGCCTCTGCTCAA
  CCTTATCCAGGTCTGCGAGGAACCAGAATTCCTGCAGAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100144 representing AP_000231
  Red=Cloning sites Green=Tags
  
  MFERKMVSFSVVVPELTCLYLHEHDYDVLSFLREALPDFLSSTLHFISPPMQQAYIGATLVSIAPSMRVI
  ISVGSFVMVPGGEVAALVRADLHDYVQLALRRDLRDRGIFVNVPLLNLIQVCEEPEFLQS
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000008
• ORF Size: 390 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000008.1, AP_000231
• RefSeq ORF: 390
• MW: 14.7 kDa