fiber (AC_000008) Virus Tagged ORF Clone

CAT#: VC100139

Myc-DDK-tagged ORF clone of viral ORF for fiber [Human adenovirus 5], codon optimized for human cell expression, AP_000226

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: fiber
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100139 represents NCBI reference of AP_000226 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCGGGCGCGGCCTAGTGAGGACACATTCAATCCTGTCTATCCGTACGATACAGAGACCGGTCCAC
  CGACCGTTCCTTTTCTGACTCCCCCTTTTGTGAGTCCCAATGGGTTTCAGGAGTCCCCTCCCGGGGTCTT
  GTCATTGCGGCTTTCCGAGCCTCTTGTGACCAGCAATGGGATGCTTGCGTTGAAAATGGGAAATGGCCTG
  AGCCTTGACGAAGCGGGTAATCTCACTTCCCAGAATGTGACGACTGTCAGCCCCCCTCTCAAGAAGACTA
  AGTCAAACATCAATCTGGAGATCAGCGCCCCCCTGACGGTCACGTCTGAGGCCTTGACCGTGGCAGCAGC
  CGCTCCTCTGATGGTCGCCGGCAACACACTGACAATGCAGTCACAAGCCCCTCTCACAGTCCACGATAGT
  AAGCTCTCTATCGCTACGCAGGGTCCACTTACTGTGTCTGAGGGAAAACTCGCCCTCCAGACCAGTGGAC
  CTCTCACCACCACCGATTCATCAACCCTGACAATCACGGCAAGCCCTCCCTTGACCACCGCCACGGGGAG
  CCTGGGCATAGACCTCAAGGAGCCTATATATACCCAGAATGGAAAGTTGGGGCTGAAGTACGGTGCCCCT
  CTGCACGTTACAGACGATCTGAATACTCTCACCGTGGCAACTGGCCCTGGAGTGACCATCAACAATACCT
  CTCTCCAGACAAAGGTGACTGGAGCTCTCGGGTTCGATTCCCAAGGTAACATGCAGCTCAACGTCGCCGG
  AGGACTGCGGATCGACAGCCAAAACCGAAGACTGATTCTCGATGTAAGTTACCCATTCGACGCACAGAAC
  CAGCTGAATCTTCGGTTGGGTCAGGGTCCCCTGTTCATCAATAGCGCCCACAACCTTGATATCAACTATA
  ACAAGGGGCTGTATTTGTTTACTGCCTCTAATAACTCCAAAAAACTGGAGGTGAATCTCAGTACTGCCAA
  GGGTCTGATGTTCGATGCAACTGCTATCGCCATCAATGCCGGCGACGGGCTGGAATTTGGATCACCAAAC
  GCTCCTAACACCAACCCCCTGAAGACTAAGATCGGGCACGGTCTGGAGTTCGACAGCAATAAGGCGATGG
  TTCCTAAGCTGGGTACTGGACTTAGCTTTGACAGCACAGGGGCGATCACAGTAGGTAATAAGAACAACGA
  CAAGCTTACACTGTGGACAACGCCAGCCCCATCCCCTAACTGTAGATTGAACGCCGAGAAGGATGCAAAA
  CTGACCCTGGTATTGACCAAATGTGGCTCTCAGATCCTGGCGACCGTCAGCGTCCTTGCAGTTAAGGGCT
  CCCTGGCACCTATAAGCGGTACAGTGCAGTCCGCCCACCTGATAATACGCTTTGATGAGAATGGTGTTCT
  CCTTAATAACTCTTTTCTCGATCCTGAATATTGGAATTTCCGCAATGGAGATCTGACCGAAGGCACCGCG
  TACACAAATGCAGTGGGTTTCATGCCCAACCTGTCTGCATACCCGAAGTCCCATGGAAAGACGGCGAAAA
  GCAATATAGTCAGCCAGGTCTACCTGAACGGAGATAAGACCAAGCCCGTAACACTCACCATCACGCTTAA
  TGGCACTCAAGAGACTGGAGACACCACTCCAAGCGCCTACAGTATGAGCTTCTCTTGGGATTGGTCAGGA
  CACAACTATATCAATGAAATCTTCGCTACCAGTTCATACACCTTCTCCTATATCGCCCAGGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100139 representing AP_000226
  Red=Cloning sites Green=Tags
  
  MKRARPSEDTFNPVYPYDTETGPPTVPFLTPPFVSPNGFQESPPGVLSLRLSEPLVTSNGMLALKMGNGL
  SLDEAGNLTSQNVTTVSPPLKKTKSNINLEISAPLTVTSEALTVAAAAPLMVAGNTLTMQSQAPLTVHDS
  KLSIATQGPLTVSEGKLALQTSGPLTTTDSSTLTITASPPLTTATGSLGIDLKEPIYTQNGKLGLKYGAP
  LHVTDDLNTLTVATGPGVTINNTSLQTKVTGALGFDSQGNMQLNVAGGLRIDSQNRRLILDVSYPFDAQN
  QLNLRLGQGPLFINSAHNLDINYNKGLYLFTASNNSKKLEVNLSTAKGLMFDATAIAINAGDGLEFGSPN
  APNTNPLKTKIGHGLEFDSNKAMVPKLGTGLSFDSTGAITVGNKNNDKLTLWTTPAPSPNCRLNAEKDAK
  LTLVLTKCGSQILATVSVLAVKGSLAPISGTVQSAHLIIRFDENGVLLNNSFLDPEYWNFRNGDLTEGTA
  YTNAVGFMPNLSAYPKSHGKTAKSNIVSQVYLNGDKTKPVTLTITLNGTQETGDTTPSAYSMSFSWDWSG
  HNYINEIFATSSYTFSYIAQE
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000008
• ORF Size: 1743 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000008.1, AP_000226
• RefSeq ORF: 1743
• MW: 61.6 kDa