10.5 kD protein (AC_000008) Virus Tagged ORF Clone

CAT#: VC100134

Myc-DDK-tagged ORF clone of viral ORF for 105 kD protein [Human adenovirus 5], codon optimized for human cell expression, AP_000221

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 10.5 kD protein
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100134 represents NCBI reference of AP_000221 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAAATACAACGAACGCCGCCGCTGCTACTGGCTTGACTTCTACAACCAACACCCCCCAGGTGTCCG
  CATTTGTGAATAATTGGGACAATCTGGGCATGTGGTGGTTCAGTATCGCCCTGATGTTTGTGTGTCTCAT
  TATCATGTGGTTGATATGTTGCCTCAAGAGGAAGCGCGCAAGGCCGCCGATTTATAGTCCCATTATAGTC
  CTCCATCCCAATAACGACGGTATCCACAGACTTGACGGGCTGAAACACATGTTTTTCTCTTTGACAGTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100134 representing AP_000221
  Red=Cloning sites Green=Tags
  
  MTNTTNAAAATGLTSTTNTPQVSAFVNNWDNLGMWWFSIALMFVCLIIMWLICCLKRKRARPPIYSPIIV
  LHPNNDGIHRLDGLKHMFFSLTV
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000008
• ORF Size: 279 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000008.1, AP_000221
• RefSeq ORF: 279
• MW: 10.5 kDa