pVI (AC_000008) Virus Tagged ORF Clone

CAT#: VC100123

Myc-DDK-tagged ORF clone of viral ORF for pVI [Human adenovirus 5], codon optimized for human cell expression, AP_000210

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: pVI
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100123 represents NCBI reference of AP_000210 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGATATTAATTTCGCCAGTCTGGCGCCCCGCCACGGTAGCCGACCTTTTATGGGCAACTGGCAGG
  ACATCGGCACTAGTAACATGAGCGGCGGTGCCTTCTCATGGGGTTCCCTGTGGAGTGGCATTAAGAATTT
  TGGCTCAACGGTGAAGAACTACGGTAGTAAGGCTTGGAACTCTAGTACGGGCCAGATGCTGAGAGACAAG
  CTGAAGGAGCAAAACTTTCAGCAGAAGGTGGTGGACGGCCTGGCTTCTGGAATCAGCGGTGTCGTCGACC
  TCGCAAATCAAGCAGTGCAGAATAAAATCAATTCCAAGTTGGACCCAAGACCTCCCGTTGAGGAACCTCC
  CCCCGCCGTGGAAACTGTTAGCCCCGAGGGCAGGGGCGAGAAAAGACCCCGGCCTGACAGGGAAGAGACT
  CTGGTCACTCAGATAGACGAGCCACCATCATATGAAGAAGCACTTAAGCAGGGCCTGCCCACAACGAGGC
  CAATCGCACCCATGGCTACTGGAGTGCTGGGCCAGCATACTCCCGTGACTCTGGATCTCCCACCCCCAGC
  GGACACCCAACAGAAGCCTGTTCTTCCAGGACCCACCGCAGTAGTTGTTACCAGACCTAGTCGGGCGAGT
  CTGAGGCGCGCCGCCAGCGGTCCAAGGTCCCTGCGCCCAGTGGCCAGCGGTAATTGGCAGTCAACCCTTA
  ATTCAATTGTAGGACTGGGCGTTCAGTCTCTGAAGCGAAGGCGGTGCTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100123 representing AP_000210
  Red=Cloning sites Green=Tags
  
  MEDINFASLAPRHGSRPFMGNWQDIGTSNMSGGAFSWGSLWSGIKNFGSTVKNYGSKAWNSSTGQMLRDK
  LKEQNFQQKVVDGLASGISGVVDLANQAVQNKINSKLDPRPPVEEPPPAVETVSPEGRGEKRPRPDREET
  LVTQIDEPPSYEEALKQGLPTTRPIAPMATGVLGQHTPVTLDLPPPADTQQKPVLPGPTAVVVTRPSRAS
  LRRAASGPRSLRPVASGNWQSTLNSIVGLGVQSLKRRRCF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000008
• ORF Size: 750 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000008.1, AP_000210
• RefSeq ORF: 750
• MW: 27.0 kDa