V (AC_000008) Virus Tagged ORF Clone

CAT#: VC100121

Myc-DDK-tagged ORF clone of viral ORF for V [Human adenovirus 5], codon optimized for human cell expression, AP_000208

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: V
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100121 represents NCBI reference of AP_000208 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTAAACGAAAAATCAAAGAGGAGATGCTCCAGGTCATCGCTCCCGAGATCTATGGACCCCCGAAAA
  AGGAGGAGCAAGATTATAAGCCACGGAAGTTGAAGAGAGTGAAGAAGAAGAAAAAGGATGACGATGATGA
  GCTTGACGACGAAGTGGAACTGCTCCACGCCACGGCTCCGCGCAGACGAGTACAGTGGAAAGGCCGCAGG
  GTCAAAAGAGTGCTGAGACCAGGGACTACTGTGGTGTTCACACCCGGGGAGAGAAGTACAAGGACATACA
  AAAGGGTATACGACGAAGTCTACGGGGACGAAGATCTGCTTGAGCAGGCAAATGAGAGATTGGGAGAGTT
  CGCCTATGGCAAGCGCCACAAGGATATGCTGGCACTGCCCCTGGACGAAGGTAACCCCACCCCCAGCCTT
  AAGCCCGTGACACTCCAGCAGGTGCTGCCAGCACTTGCGCCTAGTGAGGAGAAGCGCGGACTCAAAAGGG
  AGAGTGGCGACCTTGCGCCTACAGTGCAACTGATGGTGCCAAAGAGGCAAAGACTTGAAGACGTGTTGGA
  GAAAATGACCGTGGAACCAGGGCTGGAACCAGAAGTGCGAGTTAGGCCAATTAAACAGGTTGCACCTGGC
  CTGGGGGTCCAGACAGTCGATGTGCAGATCCCTACCACCAGCTCCACTTCTATCGCCACAGCTACCGAAG
  GGATGGAGACTCAGACCTCACCCGTGGCTTCCGCCGTGGCGGATGCAGCAGTCCAGGCCGTTGCCGCTGC
  GGCTAGCAAAACATCTACAGAAGTACAAACAGATCCATGGATGTTCAGGGTCAGCGCACCTCGCAGACCC
  AGAGGGTCCCGCAAGTATGGGGCCGCCTCCGCACTGCTTCCAGAGTACGCTTTGCATCCTTCCATCGCCC
  CCACCCCTGGCTATAGAGGGTATACCTACCGGCCACGAAGACGCGCGACCACAAGGAGGAGAACGACAAC
  AGGCACCAGGAGAAGACGGAGGCGGAGGCAGCCCGTGCTGGCTCCCATCTCAGTCAGACGGGTGGCTCGG
  GAGGGCGGGCGAACTCTGGTGCTGCCGACAGCACGGTATCATCCAAGCATAGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100121 representing AP_000208
  Red=Cloning sites Green=Tags
  
  MSKRKIKEEMLQVIAPEIYGPPKKEEQDYKPRKLKRVKKKKKDDDDELDDEVELLHATAPRRRVQWKGRR
  VKRVLRPGTTVVFTPGERSTRTYKRVYDEVYGDEDLLEQANERLGEFAYGKRHKDMLALPLDEGNPTPSL
  KPVTLQQVLPALAPSEEKRGLKRESGDLAPTVQLMVPKRQRLEDVLEKMTVEPGLEPEVRVRPIKQVAPG
  LGVQTVDVQIPTTSSTSIATATEGMETQTSPVASAVADAAVQAVAAAASKTSTEVQTDPWMFRVSAPRRP
  RGSRKYGAASALLPEYALHPSIAPTPGYRGYTYRPRRRATTRRRTTTGTRRRRRRRQPVLAPISVRRVAR
  EGGRTLVLPTARYHPSIV
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000008
• ORF Size: 1104 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000008.1, AP_000208
• RefSeq ORF: 1104
• MW: 41.4 kDa