IVa2 (AC_000008) Virus Tagged ORF Clone

CAT#: VC100114

Myc-DDK-tagged ORF clone of viral ORF for IVa2 [Human adenovirus 5], codon optimized for human cell expression, AP_000201

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: IVa2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100114 represents NCBI reference of AP_000201 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAACTCGGGGCCGCAGGCCCGCAGCCCTTCAACACCAGCAGGACCAACCTCAGGCTCATCCCGGCC
  AGCGCGCAGCTAGAAGTGCACCTCTCCATCGCGATCCTGATTATGCCGACGAAGACCCCGCCCCAGTGGA
  AAGGCACGATCCCGGACCTTCTGGCAGGGCGCCCACAACTGCCGTGCAACGGAAGCCTCCCCAACCTGCG
  AAGAGGGGTGATATGCTCGATAGGGACGCCGTGGAGCAGGTGACCGAGCTTTGGGATCGCCTGGAGCTGT
  TGGGTCAGACTCTTAAAAGCATGCCCACTGCCGACGGTCTCAAACCTCTCAAAAACTTTGCCAGTCTGCA
  GGAACTGCTGAGCTTGGGAGGGGAAAGGCTGTTGGCTGACCTGGTGAGGGAGAATATGCGCGTCAGAGAC
  ATGCTTAACGAAGTGGCTCCCCTGCTGAGGGACGACGGAAGCTGCTCCTCACTGAATTACCAGCTTCACC
  CCGTGATCGGCGTTATCTACGGCCCAACAGGCTGCGGCAAGAGCCAGCTCCTTAGGAATCTTCTGTCTTC
  CCAACTGATCAGCCCCACTCCTGAGACCGTCTTCTTTATCGCTCCTCAGGTTGACATGATCCCCCCATCT
  GAGCTTAAGGCGTGGGAGATGCAGATCTGTGAGGGTAATTATGCACCCGGGCCAGATGGAACCATTATCC
  CACAGAGTGGTACTTTGCGGCCGCGGTTCGTGAAGATGGCCTATGACGATCTGATACTCGAACATAATTA
  CGATGTCTCCGATCCGCGGAACATCTTTGCCCAAGCAGCTGCCCGCGGACCTATTGCAATTATTATGGAT
  GAGTGTATGGAGAATCTTGGGGGACATAAGGGAGTGAGTAAATTTTTCCACGCATTCCCCAGTAAGCTTC
  ATGACAAGTTTCCCAAGTGTACCGGATACACTGTTCTCGTCGTACTGCATAACATGAACCCCCGCCGCGA
  TATGGCCGGAAATATTGCGAACCTGAAGATTCAGAGCAAAATGCATCTGATCAGTCCGCGGATGCACCCA
  AGCCAGCTGAATCGATTTGTAAATACATACACCAAAGGACTGCCACTCGCGATCTCTCTCCTTCTCAAGG
  ACATTTTCCGCCATCACGCCCAACGCTCATGTTACGATTGGATTATCTACAACACCACGCCTCAGCATGA
  GGCCCTCCAATGGTGCTACTTGCATCCCAGAGATGGTCTGATGCCTATGTATCTGAACATTCAGTCACAC
  CTCTATCATGTACTTGAAAAAATCCACAGAACTCTGAACGACAGGGACAGGTGGTCCCGGGCATATAGGG
  CTAGAAAAACCCCCAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100114 representing AP_000201
  Red=Cloning sites Green=Tags
  
  METRGRRPAALQHQQDQPQAHPGQRAARSAPLHRDPDYADEDPAPVERHDPGPSGRAPTTAVQRKPPQPA
  KRGDMLDRDAVEQVTELWDRLELLGQTLKSMPTADGLKPLKNFASLQELLSLGGERLLADLVRENMRVRD
  MLNEVAPLLRDDGSCSSLNYQLHPVIGVIYGPTGCGKSQLLRNLLSSQLISPTPETVFFIAPQVDMIPPS
  ELKAWEMQICEGNYAPGPDGTIIPQSGTLRPRFVKMAYDDLILEHNYDVSDPRNIFAQAAARGPIAIIMD
  ECMENLGGHKGVSKFFHAFPSKLHDKFPKCTGYTVLVVLHNMNPRRDMAGNIANLKIQSKMHLISPRMHP
  SQLNRFVNTYTKGLPLAISLLLKDIFRHHAQRSCYDWIIYNTTPQHEALQWCYLHPRDGLMPMYLNIQSH
  LYHVLEKIHRTLNDRDRWSRAYRARKTPK
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000008
• ORF Size: 1347 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000008.1, AP_000201
• RefSeq ORF: 1347
• MW: 50.9 kDa