100K (AC_000008) Virus Tagged ORF Clone

CAT#: VC100127

Myc-DDK-tagged ORF clone of viral ORF for 100K [Human adenovirus 5], codon optimized for human cell expression, AP_000214

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 100K
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100127 represents NCBI reference of AP_000214 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAGCGTCGAGAAAAAGGACAGTTTGACCGCACCGAGTGAATTCGCAACAACCGCCAGCACTGATG
  CCGCAAATGCTCCTACAACCTTCCCCGTGGAAGCGCCTCCCCTCGAGGAAGAGGAGGTTATCATCGAGCA
  GGACCCCGGGTTCGTCAGCGAGGACGACGAGGATCGCTCTGTGCCTACAGAAGACAAGAAACAGGACCAG
  GATAACGCAGAAGCCAATGAAGAGCAGGTTGGCCGCGGTGACGAGAGGCACGGTGACTACCTGGATGTTG
  GGGATGACGTATTGTTGAAACATCTGCAGCGGCAGTGTGCCATCATCTGCGACGCCCTCCAGGAACGGAG
  CGACGTGCCCTTGGCTATAGCCGATGTGTCATTGGCTTACGAGCGCCACCTCTTTAGCCCCCGGGTCCCC
  CCAAAAAGACAGGAAAATGGCACCTGCGAACCCAATCCAAGGCTGAACTTTTATCCTGTGTTTGCCGTGC
  CCGAGGTATTGGCTACTTACCACATCTTTTTCCAGAATTGCAAAATCCCCCTGAGCTGCAGAGCTAATAG
  GAGCCGGGCAGACAAGCAGCTTGCTCTCCGACAGGGAGCAGTCATTCCAGATATAGCCTCCCTGAATGAA
  GTGCCTAAAATATTTGAGGGGCTCGGCCGAGATGAAAAACGAGCCGCAAATGCCCTCCAGCAGGAAAACT
  CCGAGAATGAATCCCACAGCGGGGTGCTGGTGGAGTTGGAGGGGGACAACGCGCGGCTGGCGGTACTGAA
  GAGGTCAATAGAAGTTACCCACTTCGCCTATCCAGCATTGAACCTGCCCCCTAAGGTGATGTCTACGGTG
  ATGAGTGAGCTTATCGTGCGCCGCGCGCAGCCTCTTGAAAGGGACGCTAACCTCCAGGAGCAGACTGAGG
  AAGGACTTCCCGCCGTGGGAGATGAGCAGCTGGCTAGATGGCTGCAGACACGCGAGCCGGCTGACTTGGA
  GGAAAGGCGGAAACTGATGATGGCCGCCGTGCTGGTTACTGTTGAGCTGGAGTGTATGCAGAGGTTTTTC
  GCAGACCCTGAGATGCAGCGCAAGCTGGAGGAGACCCTTCACTACACATTCAGACAAGGGTACGTGAGGC
  AGGCCTGCAAGATCTCAAATGTTGAATTGTGCAATCTCGTCTCCTACCTCGGAATCCTGCACGAGAATAG
  ACTGGGCCAGAATGTGCTTCACTCTACACTCAAGGGTGAAGCTAGGCGCGATTACGTCAGAGACTGTGTC
  TACCTCTTCCTGTGCTACACGTGGCAGACAGCCATGGGGGTGTGGCAGCAGTGTCTGGAAGAATGCAACC
  TGAAGGAGCTCCAAAAACTTCTTAAACAGAACCTCAAGGATCTGTGGACCGCCTTCAATGAACGAAGCGT
  CGCTGCCCATCTCGCTGATATAATCTTCCCAGAAAGGCTGCTGAAAACGCTCCAACAGGGCCTCCCAGAT
  TTCACATCCCAGAGTATGCTGCAAAATTTCAGAAACTTCATTTTGGAGCGATCCGGAATCCTGCCTGCGA
  CTTGTTGCGCTCTGCCAAGCGATTTCGTACCTATCAAGTATCGGGAGTGTCCTCCCCCTCTCTGGGGCCA
  CTGTTACCTTCTGCAACTTGCTAATTATCTCGCCTACCACTCTGACATCATGGAAGACGTGAGCGGGGAC
  GGGCTGCTGGAGTGCCACTGTAGGTGCAACCTGTGCACCCCGCATCGGAGCCTCGTCTGTAACAGTCAGC
  TTTTGAATGAGTCTCAGATTATTGGAACGTTTGAATTGCAAGGTCCAAGCCCCGACGAAAAAAGTGCCGC
  CCCAGGACTCAAACTCACGCCCGGCCTGTGGACCAGTGCCTATCTCCGCAAGTTTGTACCTGAGGACTAT
  CATGCTCACGAAATAAGATTTTATGAAGATCAGAGTCGCCCACCTAATGCCGAGCTGACCGCCTGTGTAA
  TTACCCAGGGGCACATTTTGGGCCAGCTTCAGGCTATTAACAAGGCGCGCCAGGAATTTCTGCTTAGAAA
  AGGCCGCGGAGTGTATCTGGACCCCCAGAGTGGCGAGGAGCTGAACCCCATTCCTCCTCCTCCACAGCCA
  TACCAGCAGCAGCCTAGAGCTTTGGCATCTCAGGACGGCACACAGAAAGAGGCGGCTGCTGCGGCAGCTA
  CTCATGGGCGCGGCGGCATTCTTGGTCAGAGTGGTCGAGGGGGGTTCGGAAGGGGAGGGGGTGGACACGA
  TGGACGACTGGGAGAACCCCGCAGAGGATCCTTTCGCGGTAGGAGAGGGGTAAGACGGAACACAGTTACC
  TTGGGAAGAATACCCCTTGCTGGCGCACCCGAAATAGGAAACCGCTTTCAACACGGATACAACCTGAGAT
  CCTCTGGCGCCGCCGGCACAGCTCGCAGCCCTACACAGCCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100127 representing AP_000214
  Red=Cloning sites Green=Tags
  
  MESVEKKDSLTAPSEFATTASTDAANAPTTFPVEAPPLEEEEVIIEQDPGFVSEDDEDRSVPTEDKKQDQ
  DNAEANEEQVGRGDERHGDYLDVGDDVLLKHLQRQCAIICDALQERSDVPLAIADVSLAYERHLFSPRVP
  PKRQENGTCEPNPRLNFYPVFAVPEVLATYHIFFQNCKIPLSCRANRSRADKQLALRQGAVIPDIASLNE
  VPKIFEGLGRDEKRAANALQQENSENESHSGVLVELEGDNARLAVLKRSIEVTHFAYPALNLPPKVMSTV
  MSELIVRRAQPLERDANLQEQTEEGLPAVGDEQLARWLQTREPADLEERRKLMMAAVLVTVELECMQRFF
  ADPEMQRKLEETLHYTFRQGYVRQACKISNVELCNLVSYLGILHENRLGQNVLHSTLKGEARRDYVRDCV
  YLFLCYTWQTAMGVWQQCLEECNLKELQKLLKQNLKDLWTAFNERSVAAHLADIIFPERLLKTLQQGLPD
  FTSQSMLQNFRNFILERSGILPATCCALPSDFVPIKYRECPPPLWGHCYLLQLANYLAYHSDIMEDVSGD
  GLLECHCRCNLCTPHRSLVCNSQLLNESQIIGTFELQGPSPDEKSAAPGLKLTPGLWTSAYLRKFVPEDY
  HAHEIRFYEDQSRPPNAELTACVITQGHILGQLQAINKARQEFLLRKGRGVYLDPQSGEELNPIPPPPQP
  YQQQPRALASQDGTQKEAAAAAATHGRGGILGQSGRGGFGRGGGGHDGRLGEPRRGSFRGRRGVRRNTVT
  LGRIPLAGAPEIGNRFQHGYNLRSSGAAGTARSPTQP
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000008
• ORF Size: 2421 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000008.1, AP_000214
• RefSeq ORF: 2421
• MW: 90.2 kDa