E3 gp19K (AC_000008) Virus Tagged ORF Clone

CAT#: VC100133

Myc-DDK-tagged ORF clone of viral ORF for E3 gp19K [Human adenovirus 5], codon optimized for human cell expression, AP_000220

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E3 gp19K
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100133 represents NCBI reference of AP_000220 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTAGATACATTATTCTGGGGCTGCTTACACTCGCGTCCGCGCATGGAACAACGCAGAAGGTAGACT
  TTAAGGAGCCTGCATGTAATGTAACATTTGCCGCCGAGGCGAACGAATGTACTACGCTGATTAAATGCAC
  AACAGAACACGAAAAACTCCTTATCAGACACAAAAACAAAATAGGAAAGTACGCCGTCTACGCTATTTGG
  CAGCCCGGCGATACTACAGAGTACAATGTCACTGTTTTTCAGGGAAAAAGCCACAAGACCTTCATGTATA
  CCTTCCCCTTTTACGAGATGTGCGACATTACCATGTATATGTCCAAACAGTATAAACTTTGGCCCCCTCA
  GAATTGCGTTGAAAATACTGGGACTTTCTGCTGCACAGCGATGCTCATCACCGTTCTTGCCCTGGTCTGC
  ACTTTGCTTTATATCAAATACAAATCAAGAAGGAGCTTCATCGAGGAGAAGAAGATGCCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100133 representing AP_000220
  Red=Cloning sites Green=Tags
  
  MIRYIILGLLTLASAHGTTQKVDFKEPACNVTFAAEANECTTLIKCTTEHEKLLIRHKNKIGKYAVYAIW
  QPGDTTEYNVTVFQGKSHKTFMYTFPFYEMCDITMYMSKQYKLWPPQNCVENTGTFCCTAMLITVLALVC
  TLLYIKYKSRRSFIEEKKMP
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000008
• ORF Size: 480 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000008.1, AP_000220
• RefSeq ORF: 480
• MW: 18.5 kDa