pVII (AC_000008) Virus Tagged ORF Clone

CAT#: VC100120

Myc-DDK-tagged ORF clone of viral ORF for pVII [Human adenovirus 5], codon optimized for human cell expression, AP_000207

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: pVII
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100120 represents NCBI reference of AP_000207 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCATCCTCATTAGCCCTTCAAACAATACCGGATGGGGATTGCGCTTCCCTTCTAAGATGTTTGGCG
  GTGCTAAAAAAAGAAGCGATCAGCACCCAGTCAGAGTGCGAGGACACTACAGGGCACCCTGGGGAGCCCA
  CAAGAGGGGTCGAACCGGTAGGACAACGGTGGATGATGCAATTGACGCAGTAGTAGAAGAAGCAAGGAAT
  TACACTCCCACACCACCCCCCGTGTCCACTGTTGACGCCGCAATCCAGACCGTTGTTAGGGGCGCTCGGC
  GCTACGCTAAAATGAAGCGGCGGAGGCGCAGAGTGGCACGGCGGCATAGGCGGAGGCCAGGTACTGCCGC
  TCAAAGAGCGGCAGCAGCGCTGCTGAACAGGGCTAGAAGGACTGGTAGGAGAGCCGCAATGAGAGCGGCT
  AGACGGCTCGCCGCCGGGATAGTCACAGTGCCACCAAGGAGTCGGCGAAGGGCTGCCGCAGCAGCTGCTG
  CCGCTATCAGCGCCATGACCCAGGGGAGAAGGGGGAATGTGTACTGGGTCCGGGACAGCGTGAGCGGACT
  CCGGGTGCCTGTCCGGACTAGGCCTCCTCGCAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100120 representing AP_000207
  Red=Cloning sites Green=Tags
  
  MSILISPSNNTGWGLRFPSKMFGGAKKRSDQHPVRVRGHYRAPWGAHKRGRTGRTTVDDAIDAVVEEARN
  YTPTPPPVSTVDAAIQTVVRGARRYAKMKRRRRRVARRHRRRPGTAAQRAAAALLNRARRTGRRAAMRAA
  RRLAAGIVTVPPRSRRRAAAAAAAAISAMTQGRRGNVYWVRDSVSGLRVPVRTRPPRN
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000008
• ORF Size: 594 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000008.1, AP_000207
• RefSeq ORF: 594
• MW: 22.0 kDa