E1A (AC_000008) Virus Tagged ORF Clone

CAT#: VC100110

Myc-DDK-tagged ORF clone of viral ORF for E1A [Human adenovirus 5], codon optimized for human cell expression, AP_000197

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E1A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100110 represents NCBI reference of AP_000197 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGCATATCATTTGCCATGGAGGCGTAATCACAGAGGAAATGGCAGCTTCCCTGCTCGACCAACTCA
  TCGAAGAGGTGCTGGCCGACAACTTGCCACCACCCTCTCACTTCGAACCGCCAACCTTGCACGAATTGTA
  CGATCTGGACGTGACCGCACCAGAGGACCCTAACGAGGAAGCAGTTAGCCAGATCTTCCCAGACAGTGTG
  ATGCTGGCAGTTCAGGAGGGGATAGATCTGCTGACGTTCCCTCCCGCTCCAGGCTCACCCGAACCACCCC
  ATCTGTCCAGACAACCCGAACAGCCAGAGCAGCGGGCACTGGGGCCAGTGAGCATGCCAAATTTGGTGCC
  GGAGGTGATCGATCTGACATGCCATGAGGCTGGCTTTCCCCCTTCCGACGACGAGGATGAAGAGGGAGAA
  GAATTCGTCCTCGACTACGTCGAACACCCAGGGCATGGCTGCCGCTCCTGTCATTATCACAGGCGGAACA
  CTGGCGATCCCGACATCATGTGTTCACTCTGCTATATGCGGACATGTGGAATGTTCGTTTACAGCCCAGT
  GTCCGAGCCAGAACCTGAACCAGAGCCCGAGCCAGAGCCCGCCAGACCTACCAGAAGGCCGAAGATGGCC
  CCTGCTATTCTGAGGCGGCCAACATCCCCCGTGTCTAGGGAGTGCAATAGCAGCACAGATAGCTGTGATT
  CTGGACCTAGCAATACCCCGCCGGAGATCCACCCTGTAGTCCCGCTCTGCCCGATTAAACCCGTCGCGGT
  GCGCGTCGGTGGGCGACGGCAGGCCGTCGAGTGCATCGAGGATCTCCTGAATGAGCCGGGCCAACCCCTT
  GATTTGAGTTGTAAACGCCCCAGGCCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100110 representing AP_000197
  Red=Cloning sites Green=Tags
  
  MRHIICHGGVITEEMAASLLDQLIEEVLADNLPPPSHFEPPTLHELYDLDVTAPEDPNEEAVSQIFPDSV
  MLAVQEGIDLLTFPPAPGSPEPPHLSRQPEQPEQRALGPVSMPNLVPEVIDLTCHEAGFPPSDDEDEEGE
  EFVLDYVEHPGHGCRSCHYHRRNTGDPDIMCSLCYMRTCGMFVYSPVSEPEPEPEPEPEPARPTRRPKMA
  PAILRRPTSPVSRECNSSTDSCDSGPSNTPPEIHPVVPLCPIKPVAVRVGGRRQAVECIEDLLNEPGQPL
  DLSCKRPRP
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000008
• ORF Size: 867 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000008.1, AP_000197
• RefSeq ORF: 867
• MW: 31.9 kDa