E4 ORF6/7 (AC_000008) Virus Tagged ORF Clone

CAT#: VC100140

Myc-DDK-tagged ORF clone of viral ORF for E4 ORF6/7 [Human adenovirus 5], codon optimized for human cell expression, AP_000227

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E4 ORF6/7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100140 represents NCBI reference of AP_000227 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAACCAGTGGCGTGCCCTTCGGCATGACACTGAGACCTACACGGAGCAGGCTGAGTCGGCGAACCC
  CTTATTCTCGGGATCGCCTGCCCCCATTTGAGACCGAGACACGAGCTACTATCCTGGAAGATCATCCTCT
  CTTGCCAGAATGCAACACCTTGACTATGCACAACGCTTGGACTTCACCTTCACCCCCCGTTAAACAGCCG
  CAGGTGGGCCAGCAGCCCGTCGCCCAGCAGTTGGATTCTGACATGAACCTGTCTGAGCTCCCGGGAGAAT
  TTATCAACATTACAGATGAACGGCTCGCTCGGCAAGAGACGGTGTGGAACATCACACCCAAAAACATGTC
  TGTCACCCACGATATGATGCTTTTCAAGGCAAGTCGGGGAGAGAGGACCGTGTATTCAGTCTGTTGGGAG
  GGCGGAGGTAGATTGAACACTAGAGTGTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100140 representing AP_000227
  Red=Cloning sites Green=Tags
  
  MTTSGVPFGMTLRPTRSRLSRRTPYSRDRLPPFETETRATILEDHPLLPECNTLTMHNAWTSPSPPVKQP
  QVGQQPVAQQLDSDMNLSELPGEFINITDERLARQETVWNITPKNMSVTHDMMLFKASRGERTVYSVCWE
  GGGRLNTRVL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000008
• ORF Size: 450 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000008.1, AP_000227
• RefSeq ORF: 450
• MW: 17.1 kDa