pTP (AC_000008) Virus Tagged ORF Clone

CAT#: VC100116

Myc-DDK-tagged ORF clone of viral ORF for pTP [Human adenovirus 5], codon optimized for human cell expression, AP_000203

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: pTP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100116 represents NCBI reference of AP_000203 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCTGAGCGTGAACGACTGTGCACGGTTGACCGGCCAGTCTGTGCCAACAATGGAACATTTTCTTC
  CCTTGAGGAACATCTGGAACAGAGTGAGAGACTTTCCCCGAGCCTCAACAACTGCTGCTGGCATTACCTG
  GATGTCACGATATATATATGGATACCACAGGCTCATGCTGGAGGATCTGGCTCCGGGAGCCCCTGCTACA
  CTGCGCTGGCCCCTGTATCGGCAGCCCCCCCCTCACTTCCTCGTCGGCTATCAGTACCTGGTCCGAACTT
  GTAACGATTACGTCTTTGACAGCAGGGCCTACTCAAGACTTCGCTACACTGAACTGAGCCAGCCAGGCCA
  TCAGACCGTGAATTGGTCCGTGATGGCCAACTGCACTTACACGATAAATACCGGAGCATACCACAGATTT
  GTAGACATGGACGATTTTCAGAGCACGCTGACACAGGTGCAGCAGGCTATCCTCGCGGAGAGAGTGGTAG
  CAGATCTCGCACTGCTCCAACCGATGAGGGGTTTTGGAGTTACACGCATGGGGGGTCGGGGAAGGCACCT
  TCGGCCAAATTCCGCAGCTGCTGCCGCCATCGATGCCAGGGATGCCGGTCAGGAAGAAGGCGAGGAGGAA
  GTGCCAGTTGAGAGGCTGATGCAAGACTATTATAAAGATCTTAGGCGGTGCCAGAACGAAGCATGGGGCA
  TGGCAGACCGCCTGCGGATTCAGCAAGCAGGCCCGAAGGATATGGTGCTGCTCTCCACCATCAGGCGGCT
  TAAAACAGCATACTTTAATTACATCATTTCTAGTACATCTGCTAGAAACAATCCTGATAGGCGCCCCCTT
  CCACCCGCAACAGTTCTTAGTCTCCCATGCGATTGTGACTGGCTGGATGCCTTCCTGGAAAGATTCAGCG
  ATCCTGTGGATGCTGACTCCCTCCGGTCTTTGGGGGGTGGAGTGCCAACTCAGCAGCTCTTGAGGTGTAT
  CGTATCCGCTGTGAGCCTGCCCCACGGATCACCCCCACCCACTCACAATCGGGATATGACGGGAGGAGTT
  TTTCAACTTCGGCCTCGGGAAAACGGCCGGGCAGTGACTGAGACAATGCGCCGGAGACGGGGCGAGATGA
  TTGAAAGGTTTGTGGACCGGCTGCCCGTAAGAAGGAGACGAAGAAGAGTGCCGCCTCCCCCCCCCCCCCC
  TGAAGAGGAGGAAGGTGAAGCCCTTATGGAAGAGGAGATCGAGGAAGAGGAGGAAGCGCCGGTGGCCTTC
  GAAAGGGAAGTCCGCGACACCGTCGCGGAACTCATTAGACTCCTGGAGGAAGAACTGACCGTGAGTGCTA
  GAAACTCTCAGTTCTTTAACTTTGCCGTGGATTTCTATGAGGCTATGGAGAGACTCGAGGCACTGGGCGA
  TATCAACGAATCAACCCTGAGGCGCTGGGTCATGTACTTTTTTGTGGCCGAGCATACAGCAACTACCCTG
  AACTATCTCTTCCAGAGGCTGAGAAACTATGCAGTGTTTGCAAGACATGTGGAGCTGAATCTGGCCCAGG
  TCGTAATGCGAGCCCGCGATGCAGAAGGCGGAGTTGTGTACAGCAGAGTTTGGAATGAAGGTGGTCTGAA
  CGCCTTTTCTCAGCTGATGGCACGCATCTCCAACGATCTGGCTGCTACTGTGGAGAGAGCCGGCCGGGGA
  GATTTGCAGGAAGAAGAAATCGAGCAATTTATGGCCGAGATCGCCTACCAGGATAACAGCGGTGATGTGC
  AGGAGATCCTTCGGCAGGCTGCTGTCAATGACACTGAAATCGACTCAGTTGAGCTGAGCTTTAGACTGAA
  ACTCACTGGCCCTGTGGTGTTTACACAGAGACGGCAAATTCAGGAAATCAACAGGCGAGTGGTCGCCTTT
  GCCTCCAATCTCAGAGCACAGCATCAGCTCTTGCCTGCCAGGGGCGCTGATGTTCCTCTCCCGCCACTGC
  CTGCCGGGCCTGAGCCCCCTCTCCCACCCGGAGCTCGACCCAGACATCGGTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100116 representing AP_000203
  Red=Cloning sites Green=Tags
  
  MALSVNDCARLTGQSVPTMEHFLPLRNIWNRVRDFPRASTTAAGITWMSRYIYGYHRLMLEDLAPGAPAT
  LRWPLYRQPPPHFLVGYQYLVRTCNDYVFDSRAYSRLRYTELSQPGHQTVNWSVMANCTYTINTGAYHRF
  VDMDDFQSTLTQVQQAILAERVVADLALLQPMRGFGVTRMGGRGRHLRPNSAAAAAIDARDAGQEEGEEE
  VPVERLMQDYYKDLRRCQNEAWGMADRLRIQQAGPKDMVLLSTIRRLKTAYFNYIISSTSARNNPDRRPL
  PPATVLSLPCDCDWLDAFLERFSDPVDADSLRSLGGGVPTQQLLRCIVSAVSLPHGSPPPTHNRDMTGGV
  FQLRPRENGRAVTETMRRRRGEMIERFVDRLPVRRRRRRVPPPPPPPEEEEGEALMEEEIEEEEEAPVAF
  EREVRDTVAELIRLLEEELTVSARNSQFFNFAVDFYEAMERLEALGDINESTLRRWVMYFFVAEHTATTL
  NYLFQRLRNYAVFARHVELNLAQVVMRARDAEGGVVYSRVWNEGGLNAFSQLMARISNDLAATVERAGRG
  DLQEEEIEQFMAEIAYQDNSGDVQEILRQAAVNDTEIDSVELSFRLKLTGPVVFTQRRQIQEINRRVVAF
  ASNLRAQHQLLPARGADVPLPPLPAGPEPPLPPGARPRHRF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000008
• ORF Size: 2013 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000008.1, AP_000203
• RefSeq ORF: 2013
• MW: 76.5 kDa