52K (AC_000008) Virus Tagged ORF Clone

CAT#: VC100117

Myc-DDK-tagged ORF clone of viral ORF for 52K [Human adenovirus 5], codon optimized for human cell expression, AP_000204

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 52K
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100117 represents NCBI reference of AP_000204 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACCCCGTCCTTAGACAAATGAGGCCCCCGCCTCAGCAAAGACAAGAGCAGGAGCAGCGCCAGACTT
  GTAGAGCCCCTAGTCCCCCTCCAACTGCTTCTGGCGGTGCGACTTCTGCAGTCGACGCCGCAGCCGACGG
  AGACTATGAGCCCCCTAGAAGACGGGCTCGCCATTACTTGGACCTCGAGGAGGGCGAGGGACTGGCTCGG
  CTTGGTGCACCAAGTCCAGAGAGGTACCCCCGGGTGCAGCTGAAAAGGGACACTAGAGAGGCTTATGTGC
  CTCGACAAAATCTGTTTAGGGACCGCGAGGGTGAGGAGCCAGAGGAAATGCGCGATAGAAAGTTCCACGC
  CGGCAGAGAACTGAGACACGGTCTGAATAGGGAGCGCCTTCTGAGAGAGGAAGATTTCGAGCCAGATGCA
  CGGACAGGCATTTCTCCCGCACGGGCGCACGTTGCAGCTGCTGACCTGGTCACCGCATACGAGCAGACCG
  TGAACCAGGAGATTAACTTCCAAAAGTCTTTTAACAACCACGTACGGACCCTGGTCGCCCGGGAAGAAGT
  CGCCATCGGACTGATGCACCTTTGGGACTTTGTTAGCGCGTTGGAGCAGAATCCCAACTCAAAGCCGCTC
  ATGGCCCAGCTGTTCTTGATCGTGCAGCATAGTAGAGATAACGAGGCCTTCCGCGACGCGCTTCTTAATA
  TTGTGGAGCCTGAAGGCCGGTGGCTCCTTGACCTCATCAACATTCTCCAAAGTATAGTGGTCCAGGAACG
  CAGTCTGTCTCTCGCAGATAAGGTCGCGGCCATCAACTACTCAATGTTGTCACTCGGTAAGTTTTATGCG
  AGGAAGATCTATCACACTCCCTACGTGCCCATTGATAAAGAAGTCAAGATTGAGGGGTTCTACATGAGAA
  TGGCTCTGAAGGTATTGACACTGTCCGACGATCTCGGGGTTTATCGGAACGAGCGGATCCACAAAGCCGT
  GTCTGTAAGTCGGAGGCGAGAGCTGAGCGATCGGGAACTGATGCATAGCCTTCAGCGGGCGCTCGCGGGA
  ACTGGGTCCGGAGATCGGGAGGCCGAGAGCTACTTTGACGCCGGTGCGGATCTCCGGTGGGCTCCATCAA
  GAAGAGCACTCGAAGCGGCTGGCGCTGGACCCGGCCTGGCTGTTGCTCCAGCACGGGCAGGGAACGTGGG
  CGGCGTAGAGGAGTACGATGAGGACGATGAATATGAGCCTGAAGACGGCGAGTAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100117 representing AP_000204
  Red=Cloning sites Green=Tags
  
  MHPVLRQMRPPPQQRQEQEQRQTCRAPSPPPTASGGATSAVDAAADGDYEPPRRRARHYLDLEEGEGLAR
  LGAPSPERYPRVQLKRDTREAYVPRQNLFRDREGEEPEEMRDRKFHAGRELRHGLNRERLLREEDFEPDA
  RTGISPARAHVAAADLVTAYEQTVNQEINFQKSFNNHVRTLVAREEVAIGLMHLWDFVSALEQNPNSKPL
  MAQLFLIVQHSRDNEAFRDALLNIVEPEGRWLLDLINILQSIVVQERSLSLADKVAAINYSMLSLGKFYA
  RKIYHTPYVPIDKEVKIEGFYMRMALKVLTLSDDLGVYRNERIHKAVSVSRRRELSDRELMHSLQRALAG
  TGSGDREAESYFDAGADLRWAPSRRALEAAGAGPGLAVAPARAGNVGGVEEYDEDDEYEPEDGEY
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000008
• ORF Size: 1245 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000008.1, AP_000204
• RefSeq ORF: 1245
• MW: 47.1 kDa