DBP (AC_000008) Virus Tagged ORF Clone

CAT#: VC100126

Myc-DDK-tagged ORF clone of viral ORF for DBP [Human adenovirus 5], codon optimized for human cell expression, AP_000213

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DBP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100126 represents NCBI reference of AP_000213 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTCCCGCGAGGAAGAACAAAGGGAGACCACACCCGAGCGCGGGAGAGGCGCAGCACGGCGGCCTC
  CCACTATGGAGGACGTTTCTTCACCAAGTCCATCACCTCCACCTCCCAGAGCCCCCCCCAAAAAACGCAT
  GCGGAGACGGATCGAAAGCGAAGACGAGGAGGACTCCTCTCAAGACGCCCTCGTACCCCGCACACCATCC
  CCGCGCCCTTCAACCTCTGCCGCTGATCTCGCCATCGCTCCTAAAAAGAAAAAAAAGAGGCCATCTCCGA
  AACCAGAAAGACCTCCCAGCCCGGAAGTAATAGTTGATAGTGAAGAGGAGCGAGAGGACGTTGCACTGCA
  GATGGTGGGCTTCTCTAACCCTCCCGTTCTGATTAAACACGGCAAGGGAGGCAAGCGCACTGTTCGACGA
  CTTAATGAAGATGACCCCGTGGCAAGGGGGATGCGGACTCAGGAGGAAGAAGAGGAGCCAAGCGAGGCAG
  AATCTGAGATCACCGTGATGAACCCCCTCAGCGTTCCAATCGTAAGTGCGTGGGAGAAGGGCATGGAGGC
  CGCGAGGGCTCTGATGGACAAGTATCATGTGGATAACGACCTGAAGGCTAACTTCAAGCTCCTCCCTGAT
  CAGGTTGAAGCACTGGCCGCTGTCTGCAAAACCTGGCTGAATGAGGAACACCGGGGGTTGCAGCTGACGT
  TTACCAGCAACAAAACCTTCGTGACTATGATGGGTCGATTCCTGCAGGCTTATCTGCAATCTTTCGCTGA
  GGTAACTTATAAGCACCACGAACCTACCGGATGCGCGCTCTGGCTGCACCGCTGCGCGGAAATCGAGGGC
  GAGCTGAAGTGCCTCCACGGATCAATCATGATCAACAAGGAGCATGTGATCGAAATGGATGTAACAAGTG
  AGAATGGTCAGCGCGCACTGAAGGAACAGTCAAGTAAAGCGAAGATCGTGAAAAACCGCTGGGGCCGCAA
  TGTGGTGCAAATATCCAATACCGACGCTCGCTGCTGCGTCCACGACGCCGCCTGCCCCGCTAATCAATTC
  AGCGGAAAATCCTGCGGGATGTTCTTTTCAGAGGGCGCTAAAGCACAAGTGGCCTTTAAACAAATCAAAG
  CCTTCATGCAGGCCCTGTACCCTAACGCTCAAACCGGGCACGGGCATCTCCTCATGCCACTCCGCTGTGA
  GTGCAATTCAAAACCCGGGCACGCCCCATTTCTGGGACGGCAACTTCCAAAGCTTACCCCGTTCGCCCTG
  AGTAACGCTGAGGATCTCGACGCCGACTTGATCTCAGATAAGTCCGTTCTGGCCAGCGTCCACCACCCCG
  CCCTGATCGTCTTTCAATGCTGTAATCCCGTCTACAGGAATTCTCGAGCACAGGGAGGCGGACCAAACTG
  TGATTTCAAAATCAGCGCCCCTGATCTGCTGAACGCACTGGTTATGGTGAGATCCCTGTGGTCAGAGAAT
  TTTACTGAGCTGCCCCGGATGGTTGTTCCGGAGTTTAAATGGTCAACCAAGCATCAGTACCGAAATGTCT
  CCCTGCCAGTGGCACACAGCGACGCACGGCAAAACCCATTCGACTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100126 representing AP_000213
  Red=Cloning sites Green=Tags
  
  MASREEEQRETTPERGRGAARRPPTMEDVSSPSPSPPPPRAPPKKRMRRRIESEDEEDSSQDALVPRTPS
  PRPSTSAADLAIAPKKKKKRPSPKPERPPSPEVIVDSEEEREDVALQMVGFSNPPVLIKHGKGGKRTVRR
  LNEDDPVARGMRTQEEEEEPSEAESEITVMNPLSVPIVSAWEKGMEAARALMDKYHVDNDLKANFKLLPD
  QVEALAAVCKTWLNEEHRGLQLTFTSNKTFVTMMGRFLQAYLQSFAEVTYKHHEPTGCALWLHRCAEIEG
  ELKCLHGSIMINKEHVIEMDVTSENGQRALKEQSSKAKIVKNRWGRNVVQISNTDARCCVHDAACPANQF
  SGKSCGMFFSEGAKAQVAFKQIKAFMQALYPNAQTGHGHLLMPLRCECNSKPGHAPFLGRQLPKLTPFAL
  SNAEDLDADLISDKSVLASVHHPALIVFQCCNPVYRNSRAQGGGPNCDFKISAPDLLNALVMVRSLWSEN
  FTELPRMVVPEFKWSTKHQYRNVSLPVAHSDARQNPFDF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000008
• ORF Size: 1587 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000008.1, AP_000213
• RefSeq ORF: 1587
• MW: 59.1 kDa