U exon (AC_000008) Virus Tagged ORF Clone

CAT#: VC100138

Myc-DDK-tagged ORF clone of viral ORF for U exon, partial [Human adenovirus 5], codon optimized for human cell expression, AP_000225

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U exon
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100138 represents NCBI reference of AP_000225 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAATTGTGGGCGCCGACGGCCAAGAACAGGAAGAAACCGACATCCCATTCCGGCTGTGGCGAAAGT
  TCGCTGCTAGAAGGAAGTTGCAGTACCAGTCTTGGGAAGAAGGTAAAGAAGTGCTCCTCAACAAGCTGGA
  TCGGAACCTGCTGACTGATTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100138 representing AP_000225
  Red=Cloning sites Green=Tags
  
  MKIVGADGQEQEETDIPFRLWRKFAARRKLQYQSWEEGKEVLLNKLDRNLLTDF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000008
• ORF Size: 162 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000008.1, AP_000225
• RefSeq ORF: 162
• MW: 6.5 kDa