22K (AC_000008) Virus Tagged ORF Clone

CAT#: VC100129

Myc-DDK-tagged ORF clone of viral ORF for 22K [Human adenovirus 5], codon optimized for human cell expression, AP_000216

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 22K
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100129 represents NCBI reference of AP_000216 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCACCAAAGAAAAAGCTCCAACTCCCACCACCCCCAACCGATGAAGAGGAATACTGGGACAGTCAGG
  CTGAAGAGGTCTTGGATGAAGAAGAAGAAGATATGATGGAGGATTGGGAGAGTCTGGACGAAGAGGCCTC
  TGAGGTGGAGGAGGTAAGCGACGAGACCCCCTCTCCCAGTGTCGCCTTCCCTTCTCCTGCGCCACAGAAA
  AGCGCCACCGGGAGTAGCATGGCCACAACATCTGCACCACAGGCCCCGCCCGCCCTCCCGGTGCGCCGAC
  CTAATCGACGCTGGGACACAACAGGCACTCGCGCAGGCAAGTCTAAGCAGCCACCACCACTGGCCCAGGA
  ACAGCAACAGAGACAGGGATACCGATCCTGGAGAGGGCACAAAAATGCAATCGTGGCATGCCTGCAGGAT
  TGTGGCGGAAATATTTCCTTTGCCAGGCGCTTCCTCCTGTATCACCACGGCGTAGCGTTCCCTAGAAACA
  TTCTTCACTACTACAGGCACCTCTATTCACCATATTGCACTGGAGGTTCTGGCAGTGGGTCCAATTCATC
  TGGCCATACCGAAGCTAAAGCTACTGGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100129 representing AP_000216
  Red=Cloning sites Green=Tags
  
  MAPKKKLQLPPPPTDEEEYWDSQAEEVLDEEEEDMMEDWESLDEEASEVEEVSDETPSPSVAFPSPAPQK
  SATGSSMATTSAPQAPPALPVRRPNRRWDTTGTRAGKSKQPPPLAQEQQQRQGYRSWRGHKNAIVACLQD
  CGGNISFARRFLLYHHGVAFPRNILHYYRHLYSPYCTGGSGSGSNSSGHTEAKATG
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000008
• ORF Size: 588 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000008.1, AP_000216
• RefSeq ORF: 588
• MW: 21.7 kDa