pIIIa (AC_000008) Virus Tagged ORF Clone

CAT#: VC100118

Myc-DDK-tagged ORF clone of viral ORF for pIIIa [Human adenovirus 5], codon optimized for human cell expression, AP_000205

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: pIIIa
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100118 represents NCBI reference of AP_000205 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGCAGGATGCTACCGATCCAGCCGTCCGAGCGGCCCTGCAGTCTCAGCCATCCGGTCTCAACAGTA
  CCGACGATTGGCGGCAGGTAATGGACCGAATCATGAGCCTTACAGCGCGAAATCCAGATGCCTTTCGCCA
  GCAGCCTCAAGCCAACAGGCTGAGTGCGATCCTGGAAGCCGTGGTGCCCGCTAGAGCCAATCCAACCCAC
  GAGAAGGTGCTCGCAATCGTGAACGCCCTTGCCGAGAATCGCGCGATAAGGCCCGACGAAGCAGGGCTGG
  TGTATGACGCCCTGCTGCAGCGGGTTGCCAGATACAACAGCGGAAACGTCCAGACCAACTTGGATCGGCT
  GGTGGGAGACGTGAGAGAAGCCGTAGCGCAGAGAGAAAGAGCTCAACAGCAGGGCAACCTGGGTTCTATG
  GTGGCCCTCAACGCTTTCCTGTCCACACAGCCTGCCAATGTTCCAAGAGGTCAGGAGGACTATACAAACT
  TCGTGTCCGCTCTCCGCCTGATGGTGACCGAGACACCCCAGTCCGAGGTATACCAGAGCGGCCCAGATTA
  TTTTTTTCAAACATCCAGGCAAGGACTGCAAACTGTGAATCTGTCTCAGGCCTTCAAGAACCTGCAAGGG
  CTCTGGGGTGTCAGAGCCCCAACTGGCGATCGGGCTACAGTGTCTTCACTCCTCACACCCAATAGCCGAC
  TGCTGCTGCTGCTGATAGCTCCCTTTACGGACTCAGGTTCAGTTAGTCGGGACACCTATCTCGGACATCT
  CCTGACTCTCTACCGGGAGGCAATTGGCCAGGCTCACGTAGACGAGCACACTTTTCAAGAGATAACCAGC
  GTGAGTCGAGCTCTTGGGCAAGAAGATACAGGTTCATTGGAGGCTACACTGAATTATCTGCTGACCAACA
  GACGGCAGAAAATTCCTTCTCTTCATTCCCTGAATTCAGAGGAAGAGCGCATTCTCAGGTACGTACAGCA
  ATCAGTGTCCCTCAATCTGATGAGAGATGGTGTCACCCCCTCCGTGGCCCTGGACATGACAGCAAGGAAC
  ATGGAGCCAGGAATGTACGCTTCTAACCGGCCCTTCATCAACCGGCTCATGGATTACCTGCATAGAGCGG
  CTGCCGTGAACCCCGAATACTTCACCAATGCCATTCTGAATCCCCACTGGCTGCCACCCCCCGGATTTTA
  TACAGGAGGGTTCGAAGTCCCCGAAGGGAATGATGGATTCCTTTGGGATGACATAGATGATTCTGTGTTC
  AGTCCTCAGCCTCAAACGTTGCTGGAACTGCAGCAGAGAGAGCAGGCCGAGGCCGCACTGCGCAAGGAGT
  CTTTTCGCCGCCCATCCTCACTGAGCGATCTGGGGGCCGCTGCACCAAGATCAGATGCTTCTAGTCCATT
  CCCCAGCCTCATCGGCTCCCTGACATCCACACGGACCACCAGGCCAAGATTGCTGGGGGAAGAAGAATAT
  TTGAACAACTCACTCCTGCAACCACAGAGGGAAAAGAATTTGCCCCCTGCCTTTCCCAATAATGGGATCG
  AGTCCCTGGTGGATAAGATGTCTAGGTGGAAAACTTATGCACAGGAACATCGCGACGTGCCCGGCCCACG
  ACCCCCCACCCGCCGACAGAGGCATGACAGACAGAGGGGGCTGGTATGGGAGGATGATGACTCCGCGGAT
  GATTCATCCGTCCTGGATCTGGGCGGAAGTGGAAACCCATTTGCCCACCTTCGGCCCCGGCTTGGTCGGA
  TGTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100118 representing AP_000205
  Red=Cloning sites Green=Tags
  
  MMQDATDPAVRAALQSQPSGLNSTDDWRQVMDRIMSLTARNPDAFRQQPQANRLSAILEAVVPARANPTH
  EKVLAIVNALAENRAIRPDEAGLVYDALLQRVARYNSGNVQTNLDRLVGDVREAVAQRERAQQQGNLGSM
  VALNAFLSTQPANVPRGQEDYTNFVSALRLMVTETPQSEVYQSGPDYFFQTSRQGLQTVNLSQAFKNLQG
  LWGVRAPTGDRATVSSLLTPNSRLLLLLIAPFTDSGSVSRDTYLGHLLTLYREAIGQAHVDEHTFQEITS
  VSRALGQEDTGSLEATLNYLLTNRRQKIPSLHSLNSEEERILRYVQQSVSLNLMRDGVTPSVALDMTARN
  MEPGMYASNRPFINRLMDYLHRAAAVNPEYFTNAILNPHWLPPPGFYTGGFEVPEGNDGFLWDDIDDSVF
  SPQPQTLLELQQREQAEAALRKESFRRPSSLSDLGAAAPRSDASSPFPSLIGSLTSTRTTRPRLLGEEEY
  LNNSLLQPQREKNLPPAFPNNGIESLVDKMSRWKTYAQEHRDVPGPRPPTRRQRHDRQRGLVWEDDDSAD
  DSSVLDLGGSGNPFAHLRPRLGRMF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000008
• ORF Size: 1755 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000008.1, AP_000205
• RefSeq ORF: 1755
• MW: 65.3 kDa