pol (AC_000008) Virus Tagged ORF Clone

CAT#: VC100115

Myc-DDK-tagged ORF clone of viral ORF for pol [Human adenovirus 5], codon optimized for human cell expression, AP_000202

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: pol
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100115 represents NCBI reference of AP_000202 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCACTCGCTCAAGCCCACCGAGCTAGACGACTTCATGCCGAAGCCCCCGATTCAGGTGACCAGCCGC
  CCCGCAGAAGGGTTCGGCAGCAGCCGACGCGCGCTGCCCCAGCTCCCGCTAGAGCACGAAGAAGACGAGC
  ACCTGCTCCCTCACCAGGAGGTTCTGGCGCACCCCCCACCTCAGGTGGCTCTCCCGCCTCACCACTGCTG
  GACGCAAGTTCCAAAGATACCCCCGCAGCACATCGGCCACCCAGGGGGACAGTTGTGGCTCCCAGAGGCT
  GCGGCCTGTTGCAAGCAATTGATGCAGCCACCAACCAGCCACTGGAGATACGGTATCATCTCGATCTCGC
  ACGGGCACTCACAAGACTTTGCGAAGTTAATCTTCAAGAACTGCCGCCTGATCTCACCCCAAGAGAGCTG
  CAGACTATGGATTCTTCCCATTTGCGCGACGTGGTCATCAAGCTTAGACCACCTCGCGCCGACATCTGGA
  CTTTGGGCAGCCGGGGAGTGGTCGTGCGATCTACAGTCACACCCCTGGAGCAGCCCGACGGACAGGGCCA
  AGCAGCTGAAGTTGAAGATCACCAGCCTAACCCGCCCGGGGAGGGGTTGAAGTTCCCTTTGTGCTTCCTG
  GTTAGAGGGCGCCAGGTTAACCTCGTGCAGGACGTGCAACCCGTGCACCGATGTCAGTACTGCGCCAGAT
  TCTACAAGTCACAGCATGAGTGTAGTGCCAGACGCCGGGACTTCTATTTCCATCATATCAACTCCCACTC
  CAGCAATTGGTGGAGAGAAATTCAGTTTTTCCCCATAGGAAGCCATCCCAGAACTGAAAGGCTCTTCGTC
  ACTTATGACGTGGAGACCTACACTTGGATGGGGGCGTTTGGTAAACAGCTGGTTCCATTTATGCTTGTGA
  TGAAATTCGGGGGGGACGAGCCCTTGGTTACAGCCGCCCGGGATCTTGCAGCCAATTTGGGTTGGGATCG
  ATGGGAGCAGGACCCTCTCACATTTTATTGCATCACACCAGAGAAGATGGCTATTGGCCGGCAGTTTCGC
  ACCTTCCGCGACCATCTGCAGATGCTCATGGCTCGAGATCTCTGGAGCAGCTTTGTAGCTTCCAACCCTC
  ATCTGGCTGACTGGGCTCTGTCCGAGCATGGTCTGTCTTCCCCAGAGGAGCTGACTTACGAAGAACTCAA
  AAAGCTGCCCTCTATCAAAGGTATTCCCAGATTTTTGGAGCTTTACATCGTGGGGCATAACATAAATGGG
  TTCGACGAAATAGTACTGGCCGCCCAGGTGATTAATAATAGGAGCGAGGTCCCCGGTCCTTTCAGGATTA
  CTCGCAATTTTATGCCTAGAGCCGGCAAGATCCTGTTTAATGATGTGACATTTGCTCTCCCCAATCCCCG
  ATCCAAGAAACGCACAGACTTCTTGTTGTGGGAGCAGGGCGGCTGTGACGATACCGACTTCAAGTACCAA
  TACCTCAAGGTTATGGTCCGGGATACATTTGCCCTGACACACACTTCACTTAGAAAAGCTGCCCAGGCTT
  ACGCCCTTCCAGTGGAGAAAGGATGCTGTGCCTATCAGGCTGTCAATCAATTCTACATGTTGGGATCCTA
  CAGAAGCGAAGCCGACGGGTTCCCGATCCAGGAATACTGGAAGGACAGAGAAGAATTTGTGCTGAATAGG
  GAGCTGTGGAAGAAGAAAGGGCAGGATAAGTACGACATTATCAAGGAAACACTCGATTACTGCGCCCTGG
  ATGTACAGGTGACAGCGGAGCTCGTCAACAAACTGAGGGATTCATATGCTTCCTTTGTGCGAGACGCAGT
  CGGGCTCACTGATGCCTCATTTAATGTCTTTCAGAGGCCAACTATCTCAAGCAACTCCCACGCGATCTTC
  AGGCAAATAGTGTTCCGCGCTGAGCAGCCGGCCCGCAGTAATCTGGGACCCGACTTGCTGGCACCCAGTC
  ACGAGCTGTACGATTACGTGCGCGCAAGTATCAGGGGTGGTCGCTGTTATCCAACCTACCTCGGTATCCT
  GCGGGAACCACTGTATGTTTATGACATTTGCGGGATGTACGCCTCAGCCTTGACACACCCTATGCCATGG
  GGGCCACCGCTTAATCCCTACGAACGGGCTCTCGCCGCCCGAGCATGGCAGCAAGCACTTGACCTGCAGG
  GGTGTAAGATCGATTATTTCGATGCTCGGCTGCTCCCCGGGGTTTTCACAGTGGACGCAGACCCACCCGA
  CGAAACCCAACTCGACCCGCTGCCACCTTTCTGTAGCCGAAAAGGCGGGAGACTGTGTTGGACCAATGAG
  CGGCTCCGGGGAGAGGTCGCCACAAGCGTTGACCTCGTTACCCTGCATAACAGGGGTTGGAGGGTTCATC
  TGGTGCCAGATGAAAGGACAACTGTGTTTCCTGAATGGAGGTGTGTGGCTCGAGAGTACGTGCAGCTCAA
  CATCGCCGCAAAGGAAAGAGCCGATCGGGATAAAAACCAGACTCTGAGGAGCATCGCCAAACTGCTCAGT
  AACGCACTTTACGGTAGTTTTGCCACCAAGCTGGACAACAAAAAAATTGTTTTCTCTGATCAGATGGATG
  CAGCAACCTTGAAGGGGATTACCGCTGGTCAAGTCAACATTAAAAGTAGTAGCTTTCTGGAAACCGATAA
  TCTGTCCGCCGAGGTGATGCCCGCTTTCCAAAGGGAGTATTCACCACAGCAACTGGCACTTGCAGATAGT
  GACGCAGAGGAATCCGAGGATGAGAGAGCACCAACTCCATTTTATAGCCCACCTAGCGGGACCCCCGGGC
  ACGTCGCCTACACATATAAGCCCATCACTTTCCTCGATGCAGAGGAAGGAGATATGTGTCTCCACACTCT
  TGAGCGGGTAGACCCACTCGTGGATAACGACAGGTACCCATCTCATTTGGCGTCTTTCGTACTGGCCTGG
  ACGCGGGCATTTGTATCCGAATGGAGCGAGTTCCTGTATGAGGAAGACCGGGGGACACCCCTGGAAGACC
  GGCCACTCAAGAGCGTCTACGGCGATACCGATTCCCTGTTTGTCACGGAGAGGGGACACAGGCTTATGGA
  GACACGGGGAAAGAAGCGCATCAAAAAGCATGGCGGCAATCTGGTTTTCGACCCAGAGAGACCAGAACTG
  ACATGGTTGGTGGAATGCGAAACAGTTTGTGGGGCTTGCGGCGCTGACGCTTACTCACCAGAAAGTGTGT
  TCCTGGCTCCCAAGCTCTATGCCCTCAAGTCTCTGCACTGTCCAAGCTGCGGCGCCTCAAGCAAAGGAAA
  GCTGCGGGCCAAGGGACACGCTGCTGAAGGTCTGGACTATGACACAATGGTGAAATGCTATTTGGCTGAT
  GCGCAGGGGGAAGACCGACAGAGGTTTAGCACGTCCCGCACATCCCTGAAGCGCACCCTGGCTAGCGCAC
  AACCGGGCGCCCACCCCTTTACGGTTACCCAGACTACCTTGACTCGGACGCTCCGCCCTTGGAAAGATAT
  GACCCTGGCCCGGCTGGACGAACATAGGTTGCTCCCTTACAGCGAGAGCCGACCTAATCCCCGCAATGAG
  GAAATTTGTTGGATTGAGATGCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100115 representing AP_000202
  Red=Cloning sites Green=Tags
  
  MALAQAHRARRLHAEAPDSGDQPPRRRVRQQPTRAAPAPARARRRRAPAPSPGGSGAPPTSGGSPASPLL
  DASSKDTPAAHRPPRGTVVAPRGCGLLQAIDAATNQPLEIRYHLDLARALTRLCEVNLQELPPDLTPREL
  QTMDSSHLRDVVIKLRPPRADIWTLGSRGVVVRSTVTPLEQPDGQGQAAEVEDHQPNPPGEGLKFPLCFL
  VRGRQVNLVQDVQPVHRCQYCARFYKSQHECSARRRDFYFHHINSHSSNWWREIQFFPIGSHPRTERLFV
  TYDVETYTWMGAFGKQLVPFMLVMKFGGDEPLVTAARDLAANLGWDRWEQDPLTFYCITPEKMAIGRQFR
  TFRDHLQMLMARDLWSSFVASNPHLADWALSEHGLSSPEELTYEELKKLPSIKGIPRFLELYIVGHNING
  FDEIVLAAQVINNRSEVPGPFRITRNFMPRAGKILFNDVTFALPNPRSKKRTDFLLWEQGGCDDTDFKYQ
  YLKVMVRDTFALTHTSLRKAAQAYALPVEKGCCAYQAVNQFYMLGSYRSEADGFPIQEYWKDREEFVLNR
  ELWKKKGQDKYDIIKETLDYCALDVQVTAELVNKLRDSYASFVRDAVGLTDASFNVFQRPTISSNSHAIF
  RQIVFRAEQPARSNLGPDLLAPSHELYDYVRASIRGGRCYPTYLGILREPLYVYDICGMYASALTHPMPW
  GPPLNPYERALAARAWQQALDLQGCKIDYFDARLLPGVFTVDADPPDETQLDPLPPFCSRKGGRLCWTNE
  RLRGEVATSVDLVTLHNRGWRVHLVPDERTTVFPEWRCVAREYVQLNIAAKERADRDKNQTLRSIAKLLS
  NALYGSFATKLDNKKIVFSDQMDAATLKGITAGQVNIKSSSFLETDNLSAEVMPAFQREYSPQQLALADS
  DAEESEDERAPTPFYSPPSGTPGHVAYTYKPITFLDAEEGDMCLHTLERVDPLVDNDRYPSHLASFVLAW
  TRAFVSEWSEFLYEEDRGTPLEDRPLKSVYGDTDSLFVTERGHRLMETRGKKRIKKHGGNLVFDPERPEL
  TWLVECETVCGACGADAYSPESVFLAPKLYALKSLHCPSCGASSKGKLRAKGHAAEGLDYDTMVKCYLAD
  AQGEDRQRFSTSRTSLKRTLASAQPGAHPFTVTQTTLTRTLRPWKDMTLARLDEHRLLPYSESRPNPRNE
  EICWIEMP
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000008
• ORF Size: 3594 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000008.1, AP_000202
• RefSeq ORF: 3594
• MW: 135.5 kDa