E3 12.5K (AC_000008) Virus Tagged ORF Clone

CAT#: VC100131

Myc-DDK-tagged ORF clone of viral ORF for E3 125K [Human adenovirus 5], codon optimized for human cell expression, AP_000218

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E3 12.5K
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100131 represents NCBI reference of AP_000218 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGTCTGGAGAGGCTGAGCAGCTGCGGCTCAAGCACCTTGTACACTGTAGGCGGCACAAATGCTTTG
  CCAGGGACTCCGGAGAGTTCTGCTACTTCGAATTGCCAGAGGATCACATAGAGGGACCAGCCCACGGAGT
  GCGATTGACCGCTCAGGGGGAACTCGCTCGGTCTCTCATACGGGAATTCACTCAACGCCCCCTGCTGGTC
  GAACGGGATCGGGGTCCATGCGTACTGACAGTGATCTGCAACTGCCCTAACCTTGGTTTGCACCAGGACT
  TGTGTTGTCACTTGTGCGCTGAGTACAACAAATACCGGAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100131 representing AP_000218
  Red=Cloning sites Green=Tags
  
  MLSGEAEQLRLKHLVHCRRHKCFARDSGEFCYFELPEDHIEGPAHGVRLTAQGELARSLIREFTQRPLLV
  ERDRGPCVLTVICNCPNLGLHQDLCCHLCAEYNKYRN
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000008
• ORF Size: 321 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000008.1, AP_000218
• RefSeq ORF: 321
• MW: 12.3 kDa