E1B 19K (AC_000008) Virus Tagged ORF Clone

CAT#: VC100111

Myc-DDK-tagged ORF clone of viral ORF for E1B 19K [Human adenovirus 5], codon optimized for human cell expression, AP_000198

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E1B 19K
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100111 represents NCBI reference of AP_000198 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCCTGGGAGTGTCTGGAGGATTTTTCCGCTGTTCGCAATCTGCTGGAGCAGTCCAGCAATTCAA
  CATCCTGGTTCTGGAGGTTCCTGTGGGGCTCATCCCAGGCAAAACTGGTATGTAGAATTAAGGAGGACTA
  CAAATGGGAGTTCGAGGAGCTGCTGAAAAGCTGCGGTGAATTGTTTGATTCCCTGAACCTTGGGCATCAG
  GCACTCTTCCAAGAAAAGGTCATCAAGACGCTGGACTTTAGTACGCCTGGAAGGGCCGCTGCTGCTGTGG
  CATTCCTGTCATTCATCAAAGACAAATGGTCCGAGGAGACCCACCTGTCAGGCGGATATCTCCTGGACTT
  CTTGGCCATGCACTTGTGGAGGGCCGTAGTTAGACATAAGAACCGGCTGCTGCTTCTGTCCTCAGTGCGA
  CCCGCTATTATTCCTACCGAGGAACAGCAGCAGCAGCAGGAGGAGGCTCGCCGCCGGCGCCAAGAACAAT
  CCCCCTGGAATCCGAGGGCAGGGCTGGATCCGAGAGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100111 representing AP_000198
  Red=Cloning sites Green=Tags
  
  MEAWECLEDFSAVRNLLEQSSNSTSWFWRFLWGSSQAKLVCRIKEDYKWEFEELLKSCGELFDSLNLGHQ
  ALFQEKVIKTLDFSTPGRAAAAVAFLSFIKDKWSEETHLSGGYLLDFLAMHLWRAVVRHKNRLLLLSSVR
  PAIIPTEEQQQQQEEARRRRQEQSPWNPRAGLDPRE
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000008
• ORF Size: 528 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000008.1, AP_000198
• RefSeq ORF: 528
• MW: 20.6 kDa