III (AC_000008) Virus Tagged ORF Clone

CAT#: VC100119

Myc-DDK-tagged ORF clone of viral ORF for III [Human adenovirus 5], codon optimized for human cell expression, AP_000206

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: III
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100119 represents NCBI reference of AP_000206 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCCGAGCCGCAATGTACGAGGAGGGTCCCCCACCATCCTACGAGAGCGTGGTTTCCGCTGCCCCCG
  TAGCGGCCGCGTTGGGATCTCCATTTGATGCCCCACTTGATCCACCGTTTGTCCCACCCCGCTACCTGAG
  ACCCACTGGCGGAAGAAATAGTATCCGCTACTCTGAACTTGCGCCACTGTTCGACACAACACGAGTTTAC
  CTGGTTGATAATAAGTCTACCGACGTGGCATCCCTGAATTATCAGAATGACCACTCCAATTTCTTGACCA
  CTGTGATACAGAACAATGATTATAGTCCCGGGGAAGCTAGTACCCAGACAATCAATCTCGATGATCGGTC
  CCATTGGGGAGGGGACCTGAAGACAATTCTTCACACAAACATGCCCAACGTGAACGAGTTCATGTTCACG
  AACAAGTTCAAAGCTAGGGTCATGGTAAGCCGGCTGCCGACCAAGGACAACCAGGTTGAACTGAAGTATG
  AATGGGTTGAGTTTACTTTGCCCGAAGGCAACTATTCCGAAACGATGACGATAGACCTGATGAACAATGC
  AATAGTGGAGCATTATCTGAAAGTGGGGCGCCAAAATGGTGTTCTGGAGTCCGATATAGGCGTGAAGTTT
  GATACACGAAATTTCCGGCTGGGTTTCGATCCGGTGACCGGCCTCGTCATGCCCGGGGTATATACAAACG
  AGGCCTTTCATCCGGACATCATCTTGTTGCCCGGCTGCGGGGTCGACTTTACCCATAGCCGCCTGTCAAA
  TCTCCTGGGGATTCGGAAGAGACAGCCATTCCAGGAGGGGTTTCGAATCACCTATGACGATCTTGAGGGG
  GGCAATATTCCTGCCCTTCTTGACGTGGACGCTTATCAGGCTAGCCTGAAAGACGACACCGAGCAAGGAG
  GTGGAGGGGCCGGTGGCTCCAACTCCTCTGGCAGCGGTGCTGAAGAAAACTCAAATGCCGCAGCAGCTGC
  AATGCAGCCCGTTGAAGACATGAATGACCATGCGATCCGCGGTGATACCTTCGCTACCAGGGCAGAGGAG
  AAAAGAGCTGAGGCGGAGGCAGCCGCCGAAGCTGCAGCCCCGGCGGCGCAGCCCGAGGTGGAGAAGCCCC
  AGAAGAAGCCAGTGATTAAGCCACTCACTGAAGACAGTAAGAAAAGGAGTTATAACCTGATCAGTAACGA
  TAGTACATTCACACAATATCGCAGCTGGTATCTCGCGTACAACTATGGTGATCCTCAGACTGGCATCCGG
  AGCTGGACTCTCCTCTGTACCCCCGACGTGACCTGTGGGTCCGAACAAGTCTATTGGAGCCTGCCCGACA
  TGATGCAGGACCCCGTTACTTTCAGGTCCACTCGGCAAATCAGCAATTTCCCCGTGGTTGGTGCAGAGCT
  GTTGCCAGTACACTCCAAGAGTTTCTATAACGACCAGGCTGTGTACTCCCAGCTTATTAGGCAATTTACG
  TCCCTGACCCACGTGTTCAACCGGTTCCCTGAGAACCAAATCCTGGCCCGGCCACCCGCTCCAACCATCA
  CTACCGTTAGCGAGAACGTCCCCGCCTTGACGGACCATGGCACCCTTCCTCTTAGGAACTCTATTGGGGG
  AGTGCAGCGGGTCACTATCACCGACGCCCGGAGGAGAACATGCCCTTACGTTTATAAGGCTCTGGGTATC
  GTGAGCCCAAGGGTGCTTTCCAGTCGGACCTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100119 representing AP_000206
  Red=Cloning sites Green=Tags
  
  MRRAAMYEEGPPPSYESVVSAAPVAAALGSPFDAPLDPPFVPPRYLRPTGGRNSIRYSELAPLFDTTRVY
  LVDNKSTDVASLNYQNDHSNFLTTVIQNNDYSPGEASTQTINLDDRSHWGGDLKTILHTNMPNVNEFMFT
  NKFKARVMVSRLPTKDNQVELKYEWVEFTLPEGNYSETMTIDLMNNAIVEHYLKVGRQNGVLESDIGVKF
  DTRNFRLGFDPVTGLVMPGVYTNEAFHPDIILLPGCGVDFTHSRLSNLLGIRKRQPFQEGFRITYDDLEG
  GNIPALLDVDAYQASLKDDTEQGGGGAGGSNSSGSGAEENSNAAAAAMQPVEDMNDHAIRGDTFATRAEE
  KRAEAEAAAEAAAPAAQPEVEKPQKKPVIKPLTEDSKKRSYNLISNDSTFTQYRSWYLAYNYGDPQTGIR
  SWTLLCTPDVTCGSEQVYWSLPDMMQDPVTFRSTRQISNFPVVGAELLPVHSKSFYNDQAVYSQLIRQFT
  SLTHVFNRFPENQILARPPAPTITTVSENVPALTDHGTLPLRNSIGGVQRVTITDARRRTCPYVYKALGI
  VSPRVLSSRTF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000008
• ORF Size: 1713 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000008.1, AP_000206
• RefSeq ORF: 1713
• MW: 63.3 kDa