pVIII (AC_000008) Virus Tagged ORF Clone

CAT#: VC100130

Myc-DDK-tagged ORF clone of viral ORF for pVIII [Human adenovirus 5], codon optimized for human cell expression, AP_000217

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: pVIII
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100130 represents NCBI reference of AP_000217 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCAAAGAAATTCCAACTCCCTACATGTGGAGTTATCAGCCCCAAATGGGACTGGCCGCAGGTGCAG
  CTCAGGATTACAGTACTCGGATTAACTACATGTCAGCTGGACCACACATGATATCCAGAGTGAACGGGAT
  TCGAGCTCATAGGAACAGGATCTTGCTCGAACAGGCCGCCATCACAACTACCCCCAGGAACAACCTCAAT
  CCCCGGTCCTGGCCAGCAGCCCTGGTGTATCAAGAGTCCCCTGCTCCCACGACCGTTGTGCTCCCGCGCG
  ACGCCCAAGCCGAAGTGCAGATGACTAATTCTGGTGCACAGTTGGCTGGCGGGTTCCGACACCGAGTGAG
  GAGTCCCGGCCAGGGAATTACCCACCTCACGATCAGGGGAAGGGGCATTCAGCTGAATGACGAGTCCGTG
  TCTAGCAGTTTGGGGCTCAGACCCGACGGGACCTTCCAAATAGGTGGCGCCGGCAGACCATCCTTCACGC
  CCCGCCAAGCCATCCTGACTCTGCAGACTTCATCTAGTGAACCCCGCTCTGGTGGCATAGGAACGCTGCA
  ATTTATTGAAGAATTTGTGCCATCTGTTTATTTTAACCCTTTCTCAGGACCACCCGGTCACTACCCTGAT
  CAGTTCATCCCAAACTTCGACGCGGTAAAAGATAGTGCTGACGGGTATGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100130 representing AP_000217
  Red=Cloning sites Green=Tags
  
  MSKEIPTPYMWSYQPQMGLAAGAAQDYSTRINYMSAGPHMISRVNGIRAHRNRILLEQAAITTTPRNNLN
  PRSWPAALVYQESPAPTTVVLPRDAQAEVQMTNSGAQLAGGFRHRVRSPGQGITHLTIRGRGIQLNDESV
  SSSLGLRPDGTFQIGGAGRPSFTPRQAILTLQTSSSEPRSGGIGTLQFIEEFVPSVYFNPFSGPPGHYPD
  QFIPNFDAVKDSADGYD
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000008
• ORF Size: 681 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000008.1, AP_000217
• RefSeq ORF: 681
• MW: 24.7 kDa