E1B (NC_010956) Virus Tagged ORF Clone

CAT#: VC100425

Myc-DDK-tagged ORF clone of viral ORF for control protein E1B 19K [Human adenovirus D], codon optimized for human cell expression, YP_001974423

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E1B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100425 represents NCBI reference of YP_001974423 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGTCTGGACCATCCTCGCTGACTTTAGCAAGACGCGCAGGCTGGTGGAGGATTCCTCCGATGGGT
  GCTCAGGGTTTTGGCGACATTGGTTTGGTACCCCCTTGAGTAGATTGGTGTATACCGTGAAAAAGGATTA
  CAAGGAAGAATTCGAGAATATCTTCGCTGACTGCAGTGGCCTGCTGGACTCCCTGAACCTTGGGCATCAG
  TCCCTTTTCCAAGAGAGAGTATTGCACAGCCTGGATTTCAGCTCTCCCGGACGGACAACAGCAGGAGTCG
  CATTCGTCGTGTTTCTCGTGGATAAGTGGTCCCAGGACACTCAGCTGTCAAGAGGTTACATTCTGGACTT
  CGCCGCCATGCACCTGTGGCGGGCCTGGATTAGACAGAGGGGACAGAGAATTCTTAATTATTGGCTCTTG
  CAGCCGGCTGCTCCAGGGCTGCTGAGGTTGCATCGACAGACCTCCATGCTGGAGGAAGAGATGAGACAGG
  CCATGGACGAGAATCCCAGGTCCGGACTGGACCCACCCTCCGAAGAAGAGCTGGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100425 representing YP_001974423
  Red=Cloning sites Green=Tags
  
  MDVWTILADFSKTRRLVEDSSDGCSGFWRHWFGTPLSRLVYTVKKDYKEEFENIFADCSGLLDSLNLGHQ
  SLFQERVLHSLDFSSPGRTTAGVAFVVFLVDKWSQDTQLSRGYILDFAAMHLWRAWIRQRGQRILNYWLL
  QPAAPGLLRLHRQTSMLEEEMRQAMDENPRSGLDPPSEEELD
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_010956
• ORF Size: 546 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_010956.1, YP_001974423
• RefSeq ORF: 546
• Locus ID: 6386275
• MW: 21.1 kDa