L4 (NC_010956) Virus Tagged ORF Clone

CAT#: VC100443

Myc-DDK-tagged ORF clone of viral ORF for protein 33K [Human adenovirus D], codon optimized for human cell expression, YP_001974455

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100443 represents NCBI reference of YP_001974455 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAAGGAAGAAGCAAGAGCCTCTGGTGGAAGAAATGGAAGAAGAATGGGACAGTCAAGCTGAGGAGG
  ATGAATGGGAGGAAGAAACGGAGGAAGAAGAGTTGGAAGAGGTGGAGGAGGAGCAAGCTACAGAACAGCC
  CGTCGCAGCTCCATCTGCACCCGCCGCACCAGCCGTCACCGATACAACTAGTGCTGCGCCCGCGAAGCCA
  CCAAGGAGGTGGGATCGGGTGAAAGGAGATGCTAAAAAAAAGCAGGTGCGGGAGGTCGCCGGGGGTGGCC
  TGAGGATCGCCGCAAACGAACCCCTCACAACACGCGAGCTTCGCAATCGAATTTTTCCGACCCTTTATGC
  CATCTTCCAGCAGTCCCGAGGCCAACAGCAAGAGCTGAAAGTTAAGAATCGCAGTCTCCGGTCCCTCACA
  AGGAGCTGTCTGTACCATAAGAATGAGGACCAATTGCAGCGCACCCTCGAGGATGCCGAAGCGCTGTTCC
  ATAAATATTGCGCTTTGACTCTGAAGGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100443 representing YP_001974455
  Red=Cloning sites Green=Tags
  
  MPRKKQEPLVEEMEEEWDSQAEEDEWEEETEEEELEEVEEEQATEQPVAAPSAPAAPAVTDTTSAAPAKP
  PRRWDRVKGDAKKKQVREVAGGGLRIAANEPLTTRELRNRIFPTLYAIFQQSRGQQQELKVKNRSLRSLT
  RSCLYHKNEDQLQRTLEDAEALFHKYCALTLKD
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_010956
• ORF Size: 519 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_010956.1, YP_001974455
• RefSeq ORF: 519
• Locus ID: 6386277
• MW: 19.9 kDa