E3 (NC_010956) Virus Tagged ORF Clone

CAT#: VC100449

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for membrane glycoprotein E3 CR1-beta [Human adenovirus D], codon optimized for human cell expression, YP_001974460


  View other clones from "Virus" (37)

Reconstitution Protocol

USD 520.00

3 Weeks*

Size
    • 10 ug

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Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Symbol E3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC100449 represents NCBI reference of YP_001974460 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAATACTCTCACTAGCGTGGTGCTGCTGTCCCTCTTGGTAGCTTTCTCTCAGGCCGGCATCATTAATC
TGAACGTCAGCTGGGGTATGAACTTGACCCTGGTTGGTCCATCCGATCTGCCCGTCACTTGGTACGATGG
TAAGGGCATGCAATTCTGCGATGGTAATACCATCAAGAATCCCCAGATTAAGCATAGTTGCAACCAGCAG
AATCTCACCCTGTTGAACGCAGACAAGTCCCATGAACGCACCTATCTGGGCTACCGCCATGACTCCAAAG
GGAAAGTAGATTACAAAGTCACCGTCATTCCACCCCCACCAGCCACCCGGAAACCCCTGTCCGAGCCCCA
CTACGTTACAGTGACTATGGGAGATAACATTACACTCGTTGGACCCTCTGACCTTCCAGTTACCTGGTAT
GACGGGGAAGGAAACAAATTCTGTGACGGAGAGAAAGTAGAACACGCAGAATTCAATCACACATGTAATA
TTCAGAACCTTACCCTTCTTTTCGTTAATTTGACACACAACGGCGCATACATAGGATACAACAAGGACGG
CTCCGACAGGGAGCTTTATGAGGTCAGCGTGAAAACACTGTTTCAGAACGGCGCTGGACAATCCAAAGTT
GAACAGGGCAACAAGGGCAAACCCAATACTGCGCAATCTGGAGGAAAAAAGACAAAGACTGAGCACAGGA
ACCAGTCCCCAAAGCGCAAGTCCACTAACAACCTGCAGCCCACCCAGCTGTACGTCCGGCCATTTACCAA
TGTCAGTCTGACCGGCCCCCCAAATGGAAAAGTCACGTGGTACGACGGCGAGCTTAACGATCCATGTGAG
CAGAAGTATAAGCTTCGGACTTTTTGCAACCAACAAAACCTTACCCTGATCAATGTGTCATCCACCTACG
ATGGCATATACTATGGAACTGATGAAAAGGACAAGGCTAACAGATATCGGATCAAAGTGAACACCACGAA
CCACAAGACAGTTAAGATAAAACCCCATACACGCGAGCCTCCAGCCGTGCAAGAGAAACAGTTTGAACTG
CAGGACGCAGAAACAGATGAAAATGAATCAAAAATCCCTTCCGCTACTGTGGCAATCGTGGTGGGGGTCA
TCGCGGGGTTTGTAACTCTGATTATAGTCTTTATTTGCTACATTTGTTGCCGAAAGAGACCCAGGAGCTA
CAACCATATGGTAGACCCTCTGTTGAGCTTTAGCTAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC100449 representing YP_001974460
Red=Cloning sites Green=Tags

MNTLTSVVLLSLLVAFSQAGIINLNVSWGMNLTLVGPSDLPVTWYDGKGMQFCDGNTIKNPQIKHSCNQQ
NLTLLNADKSHERTYLGYRHDSKGKVDYKVTVIPPPPATRKPLSEPHYVTVTMGDNITLVGPSDLPVTWY
DGEGNKFCDGEKVEHAEFNHTCNIQNLTLLFVNLTHNGAYIGYNKDGSDRELYEVSVKTLFQNGAGQSKV
EQGNKGKPNTAQSGGKKTKTEHRNQSPKRKSTNNLQPTQLYVRPFTNVSLTGPPNGKVTWYDGELNDPCE
QKYKLRTFCNQQNLTLINVSSTYDGIYYGTDEKDKANRYRIKVNTTNHKTVKIKPHTREPPAVQEKQFEL
QDAETDENESKIPSATVAIVVGVIAGFVTLIIVFICYICCRKRPRSYNHMVDPLLSFSY

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_010956
ORF Size 1227 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Reference Data
RefSeq NC_010956.1, YP_001974460
RefSeq ORF 1227
Locus ID 6386273
MW 45.8 kDa

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