E3 (NC_010956) Virus Tagged ORF Clone

CAT#: VC100449

Myc-DDK-tagged ORF clone of viral ORF for membrane glycoprotein E3 CR1-beta [Human adenovirus D], codon optimized for human cell expression, YP_001974460

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100449 represents NCBI reference of YP_001974460 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATACTCTCACTAGCGTGGTGCTGCTGTCCCTCTTGGTAGCTTTCTCTCAGGCCGGCATCATTAATC
  TGAACGTCAGCTGGGGTATGAACTTGACCCTGGTTGGTCCATCCGATCTGCCCGTCACTTGGTACGATGG
  TAAGGGCATGCAATTCTGCGATGGTAATACCATCAAGAATCCCCAGATTAAGCATAGTTGCAACCAGCAG
  AATCTCACCCTGTTGAACGCAGACAAGTCCCATGAACGCACCTATCTGGGCTACCGCCATGACTCCAAAG
  GGAAAGTAGATTACAAAGTCACCGTCATTCCACCCCCACCAGCCACCCGGAAACCCCTGTCCGAGCCCCA
  CTACGTTACAGTGACTATGGGAGATAACATTACACTCGTTGGACCCTCTGACCTTCCAGTTACCTGGTAT
  GACGGGGAAGGAAACAAATTCTGTGACGGAGAGAAAGTAGAACACGCAGAATTCAATCACACATGTAATA
  TTCAGAACCTTACCCTTCTTTTCGTTAATTTGACACACAACGGCGCATACATAGGATACAACAAGGACGG
  CTCCGACAGGGAGCTTTATGAGGTCAGCGTGAAAACACTGTTTCAGAACGGCGCTGGACAATCCAAAGTT
  GAACAGGGCAACAAGGGCAAACCCAATACTGCGCAATCTGGAGGAAAAAAGACAAAGACTGAGCACAGGA
  ACCAGTCCCCAAAGCGCAAGTCCACTAACAACCTGCAGCCCACCCAGCTGTACGTCCGGCCATTTACCAA
  TGTCAGTCTGACCGGCCCCCCAAATGGAAAAGTCACGTGGTACGACGGCGAGCTTAACGATCCATGTGAG
  CAGAAGTATAAGCTTCGGACTTTTTGCAACCAACAAAACCTTACCCTGATCAATGTGTCATCCACCTACG
  ATGGCATATACTATGGAACTGATGAAAAGGACAAGGCTAACAGATATCGGATCAAAGTGAACACCACGAA
  CCACAAGACAGTTAAGATAAAACCCCATACACGCGAGCCTCCAGCCGTGCAAGAGAAACAGTTTGAACTG
  CAGGACGCAGAAACAGATGAAAATGAATCAAAAATCCCTTCCGCTACTGTGGCAATCGTGGTGGGGGTCA
  TCGCGGGGTTTGTAACTCTGATTATAGTCTTTATTTGCTACATTTGTTGCCGAAAGAGACCCAGGAGCTA
  CAACCATATGGTAGACCCTCTGTTGAGCTTTAGCTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100449 representing YP_001974460
  Red=Cloning sites Green=Tags
  
  MNTLTSVVLLSLLVAFSQAGIINLNVSWGMNLTLVGPSDLPVTWYDGKGMQFCDGNTIKNPQIKHSCNQQ
  NLTLLNADKSHERTYLGYRHDSKGKVDYKVTVIPPPPATRKPLSEPHYVTVTMGDNITLVGPSDLPVTWY
  DGEGNKFCDGEKVEHAEFNHTCNIQNLTLLFVNLTHNGAYIGYNKDGSDRELYEVSVKTLFQNGAGQSKV
  EQGNKGKPNTAQSGGKKTKTEHRNQSPKRKSTNNLQPTQLYVRPFTNVSLTGPPNGKVTWYDGELNDPCE
  QKYKLRTFCNQQNLTLINVSSTYDGIYYGTDEKDKANRYRIKVNTTNHKTVKIKPHTREPPAVQEKQFEL
  QDAETDENESKIPSATVAIVVGVIAGFVTLIIVFICYICCRKRPRSYNHMVDPLLSFSY
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_010956
• ORF Size: 1227 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_010956.1, YP_001974460
• RefSeq ORF: 1227
• Locus ID: 6386273
• MW: 45.8 kDa