L3 (NC_010956) Virus Tagged ORF Clone

CAT#: VC100440

Myc-DDK-tagged ORF clone of viral ORF for protease [Human adenovirus D], codon optimized for human cell expression, YP_001974454

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100440 represents NCBI reference of YP_001974454 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGGGAGCAGTGAGAGAGAGCTCGCTGCGATCGTGCGCGATCTGGGATGCGGCCCTTACTTCCTCG
  GCACCCATGATAAGCGCTTCCCCGGCTTTTTGGCTGGCGACAAACTCGCTTGTGCAATTGTGAATACGGC
  AGGAAGAGAGACAGGGGGTGTTCATTGGCTTGCCTTCGGTTGGAACCCAAGATCACGCACATGCTACATG
  TTCGACCCATTTGGCTTTTCAGACCGGCGGCTCAAGCAGATCTACAGTTTCGAGTATGAAGCCATGCTGC
  GGAGGAGTGCCCTTGCTTCTAGCCCAGACAGATGTCTCTCCCTGGAACAGTCTACCCAAACAGTCCAGGG
  GCCTGACAGCGCGGCTTGTGGTCTGTTTTGTTGTATGTTTCTTCATGCTTTCGTCCACTGGCCTGATCGG
  CCGATGGACGGAAACCCCACCATGAACCTGCTCACCGGCGTCCCAAACGGCATGCTCCAATCCCCCCAGG
  TACTCCCTACCCTGCGGCGGAATCAGGAAGAGCTGTACCGGTTTCTGGCCAGACATTCACCTTACTTCAG
  AAGCCATAGAGCTGCTATCGAGCATGCCACGGCATTCGACAAGATGAAACAGCTGCGGGTATCTCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100440 representing YP_001974454
  Red=Cloning sites Green=Tags
  
  MSGSSERELAAIVRDLGCGPYFLGTHDKRFPGFLAGDKLACAIVNTAGRETGGVHWLAFGWNPRSRTCYM
  FDPFGFSDRRLKQIYSFEYEAMLRRSALASSPDRCLSLEQSTQTVQGPDSAACGLFCCMFLHAFVHWPDR
  PMDGNPTMNLLTGVPNGMLQSPQVLPTLRRNQEELYRFLARHSPYFRSHRAAIEHATAFDKMKQLRVSQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_010956
• ORF Size: 627 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_010956.1, YP_001974454
• RefSeq ORF: 627
• Locus ID: 6386283
• MW: 23.6 kDa