E3 (NC_010956) Virus Tagged ORF Clone

CAT#: VC100446

Myc-DDK-tagged ORF clone of viral ORF for control protein E3 125K [Human adenovirus D], codon optimized for human cell expression, YP_001974432

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100446 represents NCBI reference of YP_001974432 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTCATGGGGACAGTGCCGAACTTGCCCGCCTCCGCCACCTGGACCACTGTCGCCGGCTTCGATGTT
  TTGCGCGGGAATCCTGTGGACTCATATACTTCGAATTCCCTGAAGAACACCCTAACGGTCCAGCACATGG
  CGTGCGCATCACCGTTGAAGGGACTACGGAATCCCACTTGGTCCGATTTTTCACTCAACAGCCATTCCTG
  GTCGAGAGGGATAGAGGAGCCACCACCTATACTGTTTACTGCATCTGTCCGACTCCCAAGTTGCACGAAA
  ACTTTTGTTGCACATTGTGCGGGGAATTCAACAAATCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100446 representing YP_001974432
  Red=Cloning sites Green=Tags
  
  MSHGDSAELARLRHLDHCRRLRCFARESCGLIYFEFPEEHPNGPAHGVRITVEGTTESHLVRFFTQQPFL
  VERDRGATTYTVYCICPTPKLHENFCCTLCGEFNKS
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_010956
• ORF Size: 318 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_010956.1, YP_001974432
• RefSeq ORF: 318
• Locus ID: 6386273
• MW: 12.2 kDa