E3 (NC_010956) Virus Tagged ORF Clone

CAT#: VC100446

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for control protein E3 125K [Human adenovirus D], codon optimized for human cell expression, YP_001974432


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Reconstitution Protocol

USD 400.00

3 Weeks*

Size
    • 10 ug

Product Images

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Symbol E3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC100446 represents NCBI reference of YP_001974432 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTCATGGGGACAGTGCCGAACTTGCCCGCCTCCGCCACCTGGACCACTGTCGCCGGCTTCGATGTT
TTGCGCGGGAATCCTGTGGACTCATATACTTCGAATTCCCTGAAGAACACCCTAACGGTCCAGCACATGG
CGTGCGCATCACCGTTGAAGGGACTACGGAATCCCACTTGGTCCGATTTTTCACTCAACAGCCATTCCTG
GTCGAGAGGGATAGAGGAGCCACCACCTATACTGTTTACTGCATCTGTCCGACTCCCAAGTTGCACGAAA
ACTTTTGTTGCACATTGTGCGGGGAATTCAACAAATCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC100446 representing YP_001974432
Red=Cloning sites Green=Tags

MSHGDSAELARLRHLDHCRRLRCFARESCGLIYFEFPEEHPNGPAHGVRITVEGTTESHLVRFFTQQPFL
VERDRGATTYTVYCICPTPKLHENFCCTLCGEFNKS

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_010956
ORF Size 318 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Reference Data
RefSeq NC_010956.1, YP_001974432
RefSeq ORF 318
Locus ID 6386273
MW 12.2 kDa

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.