E3 (NC_010956) Virus Tagged ORF Clone

CAT#: VC100448

Myc-DDK-tagged ORF clone of viral ORF for membrane glycoprotein E3 gp19K [Human adenovirus D], codon optimized for human cell expression, YP_001974459

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100448 represents NCBI reference of YP_001974459 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCGGGCAAACACTGTGGGGTGGCACTATGAAGGGCCTGTTGCTCATCATTCTGTCCCTGGTTGGTG
  GTGTCCTGTCCTGTCATGAACAACCACGCTGCAATATCACTACTGGTAACGAGAGATCCGTCATTTGCAC
  TGTTGTGATCAAGTGCGAGCATGAGTGTCCTCTCAACATAACATTTAAGAATCGGACGATGGGGAACGCC
  TGGGTGGGAGATTGGGAACCAGGCGACGAGCAGAACTATACAGTGACCGTTCACGGATCCGACGGAAATC
  ACACGTTTGGCTTCAAGTTCATCTTTGAGGTTATGTGTGATATTACTTTGCACGTAGCCAGACTCCATGG
  ACTCTGGCCACCTACCAAGGAGAACATGGTGGGATTCAGTCTGGCATTTGTGATCATGGCATGTTTCATG
  AGCGGCCTGTTGGTCGGTGCATTGGTGTGGTTTTTGAAGAGAAAACCTCGGTACGGTAATGAAGAAAAGG
  AAAAACTGCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100448 representing YP_001974459
  Red=Cloning sites Green=Tags
  
  MLGQTLWGGTMKGLLLIILSLVGGVLSCHEQPRCNITTGNERSVICTVVIKCEHECPLNITFKNRTMGNA
  WVGDWEPGDEQNYTVTVHGSDGNHTFGFKFIFEVMCDITLHVARLHGLWPPTKENMVGFSLAFVIMACFM
  SGLLVGALVWFLKRKPRYGNEEKEKLL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_010956
• ORF Size: 501 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_010956.1, YP_001974459
• RefSeq ORF: 501
• Locus ID: 6386273
• MW: 18.7 kDa