L2 (NC_010956) Virus Tagged ORF Clone

CAT#: VC100435

Myc-DDK-tagged ORF clone of viral ORF for core protein precursor pVII [Human adenovirus D], codon optimized for human cell expression, YP_001974450

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100435 represents NCBI reference of YP_001974450 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAATTCTTATTAGCCCTTCCAACAATACTGGATGGGGCCTGACTAGGCCTAGCACAATGTACGGCG
  GGGCTAAGAAGCGATCTCAGCAGCATCCTGTCAGGGTCCGGGGACACTTCAGAGCCCCATGGGGAGCCTA
  CAAACGCGGGAGAACTTCAACAGCCGTACGGACCACTGTGGATGACGTCATCGATAGTGTGGTAGCTGAT
  GCTCGGAATTACACACCTGCACCTAGCACCGTTGACGCTGTGATCGATAGCGTGGTGGCCGATGCTCGGG
  ATTATGCTCGGCGGAAATCAAGAAGGCGCAGAATAGCGAGGAGACACCGGTCCACACCTGCTATGAGAGC
  GGCTAGGGCACTCCTCAGGAGAGCCAGGAGGACAGGAAGACGAGCGATGATGCGAGCTGCTAGGAGGGCC
  GCTACAGCCCCTACACCAGCAGGAAGAACTAGGCGCCGAGCAGCGGCTGCTGCTGCCGCTGCAATCTCTA
  GCATGACACGCCCACGGCGCGGCAACGTATATTGGGTGAGAGACTCAGTGACAGGTGTCAGAGTGCCTGT
  CCGGACTCGCCCCCCACGGCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100435 representing YP_001974450
  Red=Cloning sites Green=Tags
  
  MSILISPSNNTGWGLTRPSTMYGGAKKRSQQHPVRVRGHFRAPWGAYKRGRTSTAVRTTVDDVIDSVVAD
  ARNYTPAPSTVDAVIDSVVADARDYARRKSRRRRIARRHRSTPAMRAARALLRRARRTGRRAMMRAARRA
  ATAPTPAGRTRRRAAAAAAAAISSMTRPRRGNVYWVRDSVTGVRVPVRTRPPRP
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_010956
• ORF Size: 582 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_010956.1, YP_001974450
• RefSeq ORF: 582
• Locus ID: 6386285
• MW: 21.6 kDa