E4 (NC_010956) Virus Tagged ORF Clone

CAT#: VC100461

Myc-DDK-tagged ORF clone of viral ORF for control protein E4orf1 [Human adenovirus D], codon optimized for human cell expression, YP_001974474

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100461 represents NCBI reference of YP_001974474 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAAAGTCTTTATGCCTTTATCGATAGTCCCGGAGGAATCGCTCCCGTGCAAGAGGGAACATCTA
  ACAGGTACACATTCTTCTGCCCCGAATCCTTTCATATACCACCCCACGGCGTGGTCCTGCTGCACCTGAA
  AGTGAGCGTCCTGGTCCCTACAGGATATCAGGGACGATTTATGGCACTGAACGATTACCACGCCCGGGAC
  ATCCTGACCCAAAGTGACGTCATATTCGCAGGGCGACGCCAGGAACTCACCGTCCTGTTGTTTAACCATA
  CCGATCGGTTTCTGTACGTGAGGAAGGGTCATCCAGTTGGAACTCTGCTTTTGGAGCGGGTGATCTTCCC
  TTCTGTCAAGATTGCGACACTGGTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100461 representing YP_001974474
  Red=Cloning sites Green=Tags
  
  MAESLYAFIDSPGGIAPVQEGTSNRYTFFCPESFHIPPHGVVLLHLKVSVLVPTGYQGRFMALNDYHARD
  ILTQSDVIFAGRRQELTVLLFNHTDRFLYVRKGHPVGTLLLERVIFPSVKIATLV
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_010956
• ORF Size: 375 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_010956.1, YP_001974474
• RefSeq ORF: 375
• Locus ID: 6386282
• MW: 14.0 kDa