L4 (NC_010956) Virus Tagged ORF Clone

CAT#: VC100445

Myc-DDK-tagged ORF clone of viral ORF for capsid protein precursor pVIII [Human adenovirus D], codon optimized for human cell expression, YP_001974457

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100445 represents NCBI reference of YP_001974457 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAAAAGAAATTCCTACTCCGTATATGTGGTCATATCAGCCTCAGATGGGCCTTGCGGCTGGGGCGT
  CCCAAGACTACTCCACTAGAATGAATTGGCTGTCTGCTGGACCCTCTATGATTTCACGGGTGAACGGAGT
  CCGCAGTCACAGAAATCAGATTCTCTTGGAGCAGGCAGCCGTTACCTCCACACCAAGAGCAAAGCTGAAT
  CCAAGGAACTGGCCATCCACACTGGTTTACCAGGAGATACCTGGCCCCACAACCGTCCTCCTGCCGCGGG
  ATGCACTCGCCGAGGTAAGGATGACGAATTCCGGAGTCCAACTTGCCGGGGGCGCCAGTAGGTGTCCTCT
  GCGACCCCAGAGCGGCATCAAAACCCTCGTCATCAGGGGGAGGGGCACACAGCTGAACGATGAACTGGTG
  TCTAGCTCAATTGGCCTTAGACCTGATGGAGTGTTTCAGCTTGCAGGAGCCGGCAGGTCTTCTTTCACTC
  CAAATCAGGCATATCTGACCCTTCAGAGTTCTTCCAGCGAGCCACGGTCCGGCGGGATCGGAACCCTCCA
  GTTCGTGGAAGAGTTTGTCCCTAGCGTATATTTCAATCCATTCTCAGGGTCCCCTGGTCTGTATCCGGAT
  GAGTTCATCCCTAATTTCGATGCAGTTCGGGAAGCTGTAGATGGCTATGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100445 representing YP_001974457
  Red=Cloning sites Green=Tags
  
  MSKEIPTPYMWSYQPQMGLAAGASQDYSTRMNWLSAGPSMISRVNGVRSHRNQILLEQAAVTSTPRAKLN
  PRNWPSTLVYQEIPGPTTVLLPRDALAEVRMTNSGVQLAGGASRCPLRPQSGIKTLVIRGRGTQLNDELV
  SSSIGLRPDGVFQLAGAGRSSFTPNQAYLTLQSSSSEPRSGGIGTLQFVEEFVPSVYFNPFSGSPGLYPD
  EFIPNFDAVREAVDGYD
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_010956
• ORF Size: 681 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_010956.1, YP_001974457
• RefSeq ORF: 681
• Locus ID: 6386277
• MW: 24.6 kDa