L1 (NC_010956) Virus Tagged ORF Clone

CAT#: VC100430

Myc-DDK-tagged ORF clone of viral ORF for protein 136K [Human adenovirus D], codon optimized for human cell expression, YP_001974467

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100430 represents NCBI reference of YP_001974467 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGAGCAGACTGGGAGGAACTTGACTTTCTGCCTCCCGTGGGCAGGGTAGCTGTAGACGTCATGAAGG
  TGGAAATACCTCCCGCTAACCGAGCTCTCGTCCTGATGTTGGTCAAAGCTTCAGCAGTGCTCGCCGCACT
  GCACGGGCTTTACCTTATACACGAGATCCACAGCGCGTCACTCGAAGAGGAGCTGCAAGAATGGCGGCCT
  TGGCTCGTTGCGTTCATGTTCGCCTGTGTCGGACTTACGCTGGGCCTGCTTGAAGATGGAGAGGCCGATG
  AACCCGCAAGGGAACCCGGCCCTGATCTGGGCGCTGCGGGCGCTGAATCAGAAGACGAGGGTGCCCAGCT
  GGGAGCTGTCCACGGCGTTGCTGAAATTCAGGGAACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100430 representing YP_001974467
  Red=Cloning sites Green=Tags
  
  MRADWEELDFLPPVGRVAVDVMKVEIPPANRALVLMLVKASAVLAALHGLYLIHEIHSASLEEELQEWRP
  WLVAFMFACVGLTLGLLEDGEADEPAREPGPDLGAAGAESEDEGAQLGAVHGVAEIQGT
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_010956
• ORF Size: 387 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_010956.1, YP_001974467
• RefSeq ORF: 387
• Locus ID: 6386276
• MW: 13.8 kDa