L2 (NC_010956) Virus Tagged ORF Clone

CAT#: VC100434

Myc-DDK-tagged ORF clone of viral ORF for penton base [Human adenovirus D], codon optimized for human cell expression, YP_001974428

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100434 represents NCBI reference of YP_001974428 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGAGGGCAGTGGTTAGCTCTTCCCCACCTCCATCTTACGAGAGTGTGATGGCACAGGCTACACTGG
  AAGTCCCCTTTGTCCCACCCAGATACATGGCGCCGACCGAAGGAAGAAATAGCATACGCTATAGTGAACT
  TGCTCCTCAATACGACACAACCCGGGTATATCTTGTGGACAACAAATCAGCAGACATCGCCTCTCTGAAT
  TATCAGAACGACCATAGTAACTTCCTGACTACCGTGGTGCAGAATAATGACTTTACCCCTGCTGAAGCCT
  CTACTCAAACGATCAATTTTGACGAGCGAAGCCGGTGGGGTGGCGACCTCAAGACTATCCTTCATACGAA
  TATGCCAAACGTGAACGAGTACATGTTTACAAGCAAGTTCAAAGCCAGAGTAATGGTGGCACGGAAACAC
  CCCGAAGGCGTGGTAGAGACCGATCTCAGCCAGGACAAACTGGAGTACGAGTGGTTTGAGTTTACCCTCC
  CCGAGGGCAACTTTAGCGAAACCATGACTATTGATCTCATGAATAACGCTATACTCGAGAACTATTTGCA
  GGTTGGAAGGCAGAATGGGGTTCTCGAGTCTGATATAGGCGTGAAGTTTGACTCTAGGAACTTTAAACTG
  GGTTGGGACCCTGTGACAAAGCTCGTGATGCCAGGCGTGTACACCTACGAAGCCTTTCACCCTGACGTGG
  TCTTGCTCCCCGGCTGTGGGGTGGATTTCACAGAGAGCAGACTCTCAAATCTGCTGGGCATTCGAAAGAA
  GCAGCCCTTCCAAGAGGGTTTCAGGATTATGTACGAGGATCTGGAGGGCGGAAACATCCCTGCATTGCTC
  GATGTGCCAAAATACCTTGAGTCTAAGAAAAAGGTGGAGGACGAGACCAAGAACGCAGCCGCCGCTACCG
  CCGATACTACCACCCGAGGTGACACATTCGCCACACCAGCTCAAGAGACCGCAGCAGATAAAAAGGTGGA
  AGTTCTGCCCATTGAGAAGGATGAGAGTGGCCGGAGCTACAACCTGATTCAGGGAACTCACGATACTCTG
  TATCGCTCATGGTATCTTTCCTACACCTACGGGGACCCAGAAAAGGGAGTCCAGAGCTGGACCCTGCTGA
  CCACGCCCGACGTGACCTGTGGCGCTGAACAAGTCTATTGGTCACTGCCGGATCTCATGCAGGACCCTGT
  GACTTTTCGCTCCACTCAACAAGTAAGTAATTACCCTGTCGTAGGAGCTGAACTGATGCCCTTCAGGGCC
  AAGTCATTTTACAACGACCTGGCTGTGTACTCTCAGTTGATTCGAAGTTACACTTCTCTGACTCACGTTT
  TCAATCGCTTTCCCGACAACCAGATTCTGTGCAGGCCCCCAGCACCCACTATAACCACTGTGTCAGAAAA
  CGTTCCCGCATTGACCGACCACGGAACGCTCCCTCTCCGCAGTAGTATACGAGGCGTCCAACGGGTGACA
  GTGACTGACGCACGAAGAAGGACATGCCCGTATGTCTATAAGGCCCTGGGTATCGTGGCACCCCGAGTCC
  TGTCATCACGCACCTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100434 representing YP_001974428
  Red=Cloning sites Green=Tags
  
  MRRAVVSSSPPPSYESVMAQATLEVPFVPPRYMAPTEGRNSIRYSELAPQYDTTRVYLVDNKSADIASLN
  YQNDHSNFLTTVVQNNDFTPAEASTQTINFDERSRWGGDLKTILHTNMPNVNEYMFTSKFKARVMVARKH
  PEGVVETDLSQDKLEYEWFEFTLPEGNFSETMTIDLMNNAILENYLQVGRQNGVLESDIGVKFDSRNFKL
  GWDPVTKLVMPGVYTYEAFHPDVVLLPGCGVDFTESRLSNLLGIRKKQPFQEGFRIMYEDLEGGNIPALL
  DVPKYLESKKKVEDETKNAAAATADTTTRGDTFATPAQETAADKKVEVLPIEKDESGRSYNLIQGTHDTL
  YRSWYLSYTYGDPEKGVQSWTLLTTPDVTCGAEQVYWSLPDLMQDPVTFRSTQQVSNYPVVGAELMPFRA
  KSFYNDLAVYSQLIRSYTSLTHVFNRFPDNQILCRPPAPTITTVSENVPALTDHGTLPLRSSIRGVQRVT
  VTDARRRTCPYVYKALGIVAPRVLSSRTF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_010956
• ORF Size: 1557 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_010956.1, YP_001974428
• RefSeq ORF: 1557
• Locus ID: 6386285
• MW: 58.6 kDa