E1B (NC_010956) Virus Tagged ORF Clone

CAT#: VC100426

Myc-DDK-tagged ORF clone of viral ORF for control protein E1B 55K [Human adenovirus D], codon optimized for human cell expression, YP_001974446

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E1B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100426 represents NCBI reference of YP_001974446 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCCGGACATCCGACTGAGCAGGGCCTGCATCCTGGCCTGCGGAGCCACGCCCCAGTCGAGGGAC
  TCGATCAGGCAGCCGGCACAGAAAATCTCGAGCTGCTTGCATCCACTGCCAGTTCAAGTGGAAGCTCTAG
  TAGTACTCAGACTAACATTCACGTCGGAGGACGAAACGAAGCCGGACACGGCCGGGAGCCAGAAGAGCGC
  CCCGGACCTTCTGTGGGAAGAGGGGCCGGTCTGAATCAGGTGTCATCTCTGTACCCAGAATTGTCTAAGG
  TTCTCACGAGCATGGCCCGGGGCGTGAAGAGAGAGAGGAGTGACGGGGGCAACACTGGGATGATGACAGA
  GTTGACTGCTAGCCTGATGAACCGCAAGAGGCCCGAGAGATTGACCTGGTACGAGTTGCAGCAAGAGTGC
  CGAGACGAGCTCGGCTTGATGCAAGATAAGTATGGTCTCGAGCAGATCAAGACACACTGGCTGAATCCAG
  ACGAGGACTGGGAGGAGGCCATTAAGAAATATGCAAAAATAGCCCTGCGCCCTGACTGCAAGTATATCGT
  GACCAAGACCGTAAATATCAGGCACGCATGCTATATTTCTGGTAATGGAGCAGAGGTGGTTATCGACACA
  TTGGACAAAGCAGCGTTCAGATGCTGCATGATGGGGATGAGAGCAGGAGTCATGAATATGAATAGCATGA
  TCTTCATGAACATGAAGTTTAATGGTGAGAAATTTAACGGAGTGCTGTTCATGGCAAACTCTCATATGAC
  CCTGCACGGGTGCTCCTTTTTTGGCTTCAACAACATGTGCGCTGAGGTCTGGGGGGCATCCAAGATTAGG
  GGCTGCAAATTCTACGGATGTTGGATGGGTGTGGTCGGACGGCCGAAAAGTGAGATGTCAGTCAAGCAGT
  GCGTGTTCGAAAAATGCTATCTGGGAGTGTCAACGGAGGGTAATGCGAGGGTGCGACACTGCTCCTCTTT
  GGAGACCGGATGTTTTTGTTTGGTGAAGGGGACAGCCAGCCTGAAGCATAATATGGTGAAGGGGTGTACA
  GACGAGCGCATGTACAATATGCTTACCTGCGACTCCGGCGTCTGTCATATATTGAAGAACATTCATGTTA
  CATCCCACCCAAGGAAGAAGTGGCCGGTCTTTGAGAATAACCTGCTGATAAAATGTCACATGCACCTGGG
  TGCACGACGGGGTACATTTCAGCCATACCAATGCAACTTCTCTCAGACTAAACTTTTGCTGGAGAATGAT
  GCATTTAGTAGGGTTAATCTCAATGGGATCTTCGACATGGATGTTTCCGTGTACAAAATCCTGCGCTATG
  ACGAGACCAAGAGTCGCGTTCGGGCTTGTGAATGCGGAGGACGCCATACCAGAATGCAGCCAGTCGCCCT
  GGACGTTACAGAGGAACTGCGCCCTGACCATCTCGTTATGGCATGTACCGGCACCGAGTTCAGCAGCAGT
  GGCGAGGACACCGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100426 representing YP_001974446
  Red=Cloning sites Green=Tags
  
  MEPGHPTEQGLHPGLRSHAPVEGLDQAAGTENLELLASTASSSGSSSSTQTNIHVGGRNEAGHGREPEER
  PGPSVGRGAGLNQVSSLYPELSKVLTSMARGVKRERSDGGNTGMMTELTASLMNRKRPERLTWYELQQEC
  RDELGLMQDKYGLEQIKTHWLNPDEDWEEAIKKYAKIALRPDCKYIVTKTVNIRHACYISGNGAEVVIDT
  LDKAAFRCCMMGMRAGVMNMNSMIFMNMKFNGEKFNGVLFMANSHMTLHGCSFFGFNNMCAEVWGASKIR
  GCKFYGCWMGVVGRPKSEMSVKQCVFEKCYLGVSTEGNARVRHCSSLETGCFCLVKGTASLKHNMVKGCT
  DERMYNMLTCDSGVCHILKNIHVTSHPRKKWPVFENNLLIKCHMHLGARRGTFQPYQCNFSQTKLLLEND
  AFSRVNLNGIFDMDVSVYKILRYDETKSRVRACECGGRHTRMQPVALDVTEELRPDHLVMACTGTEFSSS
  GEDTD
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_010956
• ORF Size: 1485 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_010956.1, YP_001974446
• RefSeq ORF: 1485
• Locus ID: 6386275
• MW: 55.2 kDa