IVa2 (NC_010956) Virus Tagged ORF Clone

CAT#: VC100428

Myc-DDK-tagged ORF clone of viral ORF for encapsidation protein IVa2 [Human adenovirus D], codon optimized for human cell expression, YP_001974425

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: IVa2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100428 represents NCBI reference of YP_001974425 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAACTCGAGGAAGGCGACCCTGTCCATTCCAGCATCAGCAGGATGAGTCACAGGCACACCCTTGCA
  AAAGGCCGGCTCGAGGACCTCCCCTGCACAGAGACGGAGACCATCCCCACTCCGACCCTGAAACTCTGGA
  GGGACATGACGCCGGGCGGGCCGGACGACCATCTAGCCGAGCTCTCCAGTCCCAGTCTAGTCAACCGCCA
  AAGAGAGGATCATTGTTGGATCGGGATGCCGTGGAGCACGTGACTGAGCTTTGGGATCGCTTGGAGCTTC
  TGTCACAGACCCTTGCCAAAATGCCAATGGCAGACGGGCTCAAACCCTTGAAGAACTTTGCCTCCCTGCA
  GGAGCTGCTGTCTTTGGGCGGTGATCGCTTGCTCGGAGAACTGGTGCGCGAGAATCTGCAGGTACGAGAC
  ATGCTTAATGAAGTTGCCCCCTTGCTGCGAGACGACGGAAGTTGTATGTCACTGAACTATCACCTTCAAC
  CCGTAATAGGAGTCATTTACGGCCCAACCGGATGTGGCAAGAGCCAACTGCTCAGGAACCTGCTGTCCTC
  ACAGCTGATTACGCCAGCCCCCGAAACAGTGTTTTTTATTGCGCCACAGGTTGATATGATTCCTCCCAGT
  GAAATGAAGGCATGGGAGATGCAAATATGTGAGGGAAATTTCGCTCCAGGACCCGAGGGTACCATCGTGC
  CACAATCAGGTACCCTTCGCCCCAAGTTCATTAAAATGAGTTACGACGACCTGACACAGGAGCATAACTA
  TGATGTGAGCGATCCACGCAACGTATTCGCCAAAGCCGCCGCCCATGGTCCTATCGCCATTATCATGGAT
  GAGTGCATGGAAAACCTTGGCGGCCATAAAGGGGTCAGCAAGTTTTTTCACGCCTTTCCAAGCAAATTGC
  ATGACAAGTTCCCTAAGTGCACAGGGTACACTGTGCTCGTTGTTCTGCACAACATGAACCCCCGCCGCGA
  TCTCGGGGGCAACATTGCGAATCTTAAAATTCAGGCGAAGCTCCATATTATATCCCCAAGAATGCATCCG
  TCCCAGCTTAATCGATTCGCCAACACGTACACCAAGGGGCTCCCAGTAGCAATTAGTTTGTTGCTCAAGG
  ACATAATCCAGCACCATGCTCAGAGGCCTTGTTACGACTGGATTATATACAATACAACCCCAGAGCACGA
  AGCCATGCAGTGGTGTTACCTGCACCCGAGGGACGGTCTCATGCCCATGTACCTGAACATCCAGAGTCAC
  CTGTATCGAGTACTTGAAAAAATACATAGGACTCTCAACGACCGGGAGCGCTGGACACGCGCATATCGCG
  CTCGAAAGAATAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100428 representing YP_001974425
  Red=Cloning sites Green=Tags
  
  METRGRRPCPFQHQQDESQAHPCKRPARGPPLHRDGDHPHSDPETLEGHDAGRAGRPSSRALQSQSSQPP
  KRGSLLDRDAVEHVTELWDRLELLSQTLAKMPMADGLKPLKNFASLQELLSLGGDRLLGELVRENLQVRD
  MLNEVAPLLRDDGSCMSLNYHLQPVIGVIYGPTGCGKSQLLRNLLSSQLITPAPETVFFIAPQVDMIPPS
  EMKAWEMQICEGNFAPGPEGTIVPQSGTLRPKFIKMSYDDLTQEHNYDVSDPRNVFAKAAAHGPIAIIMD
  ECMENLGGHKGVSKFFHAFPSKLHDKFPKCTGYTVLVVLHNMNPRRDLGGNIANLKIQAKLHIISPRMHP
  SQLNRFANTYTKGLPVAISLLLKDIIQHHAQRPCYDWIIYNTTPEHEAMQWCYLHPRDGLMPMYLNIQSH
  LYRVLEKIHRTLNDRERWTRAYRARKNK
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_010956
• ORF Size: 1344 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_010956.1, YP_001974425
• RefSeq ORF: 1344
• Locus ID: 6386270
• MW: 50.8 kDa