E3 (NC_010956) Virus Tagged ORF Clone

CAT#: VC100450

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for membrane glycoprotein E3 CR1-gamma [Human adenovirus D], codon optimized for human cell expression, YP_001974461


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Reconstitution Protocol

USD 400.00

3 Weeks*

Size
    • 10 ug

Product Images

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Symbol E3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC100450 represents NCBI reference of YP_001974461 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAAGCTTTCACAGCCTGTGTCTTGATCAGTATAGTGACACTTAGCTCCGCAGCCATGATTAATGTAA
ATGTTACCCGCGGCGGCAAGATTACTCTGAATGGAACTTATCCTCAGACAACATGGACAAGATATCATAA
AGACGGGTGGAAAAACATTTGCGAATGGAACGTGACCGCATATAAATGCTTCAATAACGGTAGTATTACA
ATCACGGCCACTGCGAACATAACATCTGGAACTTACAAGGCCGAAAGTTATAAGAACGAGATTAAGAAGC
TGACATACAAAAACAACAAAACAACTTTTGAGGACTCAGGCAACTACGAACATCAAAAACTGAGCTTTTA
CATGCTTACTATAATAGAACTGCCTACCACCAAGGCCCCAACTACTACTTCCACAACAATAAAGACTACC
ACCCACACAACTGTCCAGAATACCACATTGCTCATCGGGTTTTTGCTGCGCGAAAATGAATCTACCACAG
AGCAAACAGAGGCCACCAGCAGTGCGTTCTCATCTACAGCAAACCTGACATCTCTGGCGTGGACTAATGA
GACTGGCGTGAGCTTGATGCATGGCCAGCCCTATAGTGGGCTTGACATTCAGATAACATTTTTGGTTGTG
TGTGGCATCTTCATACTGGTGGTGCTTCTGTATTTCGTGTGCTGCAAGGCTAGGGAGAAGTCCCGCCGGC
CGATCTATAGACCAGTGATCGGAGAGCCCCAGCCACTGCAGGTTGATGGGGGCCTGCGGAATCTGCTGTT
CAGCTTCACCGTTTGG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC100450 representing YP_001974461
Red=Cloning sites Green=Tags

MKAFTACVLISIVTLSSAAMINVNVTRGGKITLNGTYPQTTWTRYHKDGWKNICEWNVTAYKCFNNGSIT
ITATANITSGTYKAESYKNEIKKLTYKNNKTTFEDSGNYEHQKLSFYMLTIIELPTTKAPTTTSTTIKTT
THTTVQNTTLLIGFLLRENESTTEQTEATSSAFSSTANLTSLAWTNETGVSLMHGQPYSGLDIQITFLVV
CGIFILVVLLYFVCCKAREKSRRPIYRPVIGEPQPLQVDGGLRNLLFSFTVW

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_010956
ORF Size 786 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Reference Data
RefSeq NC_010956.1, YP_001974461
RefSeq ORF 786
Locus ID 6386273
MW 29.2 kDa

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