E3 (NC_010956) Virus Tagged ORF Clone

CAT#: VC100450

Myc-DDK-tagged ORF clone of viral ORF for membrane glycoprotein E3 CR1-gamma [Human adenovirus D], codon optimized for human cell expression, YP_001974461

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100450 represents NCBI reference of YP_001974461 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAGCTTTCACAGCCTGTGTCTTGATCAGTATAGTGACACTTAGCTCCGCAGCCATGATTAATGTAA
  ATGTTACCCGCGGCGGCAAGATTACTCTGAATGGAACTTATCCTCAGACAACATGGACAAGATATCATAA
  AGACGGGTGGAAAAACATTTGCGAATGGAACGTGACCGCATATAAATGCTTCAATAACGGTAGTATTACA
  ATCACGGCCACTGCGAACATAACATCTGGAACTTACAAGGCCGAAAGTTATAAGAACGAGATTAAGAAGC
  TGACATACAAAAACAACAAAACAACTTTTGAGGACTCAGGCAACTACGAACATCAAAAACTGAGCTTTTA
  CATGCTTACTATAATAGAACTGCCTACCACCAAGGCCCCAACTACTACTTCCACAACAATAAAGACTACC
  ACCCACACAACTGTCCAGAATACCACATTGCTCATCGGGTTTTTGCTGCGCGAAAATGAATCTACCACAG
  AGCAAACAGAGGCCACCAGCAGTGCGTTCTCATCTACAGCAAACCTGACATCTCTGGCGTGGACTAATGA
  GACTGGCGTGAGCTTGATGCATGGCCAGCCCTATAGTGGGCTTGACATTCAGATAACATTTTTGGTTGTG
  TGTGGCATCTTCATACTGGTGGTGCTTCTGTATTTCGTGTGCTGCAAGGCTAGGGAGAAGTCCCGCCGGC
  CGATCTATAGACCAGTGATCGGAGAGCCCCAGCCACTGCAGGTTGATGGGGGCCTGCGGAATCTGCTGTT
  CAGCTTCACCGTTTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100450 representing YP_001974461
  Red=Cloning sites Green=Tags
  
  MKAFTACVLISIVTLSSAAMINVNVTRGGKITLNGTYPQTTWTRYHKDGWKNICEWNVTAYKCFNNGSIT
  ITATANITSGTYKAESYKNEIKKLTYKNNKTTFEDSGNYEHQKLSFYMLTIIELPTTKAPTTTSTTIKTT
  THTTVQNTTLLIGFLLRENESTTEQTEATSSAFSSTANLTSLAWTNETGVSLMHGQPYSGLDIQITFLVV
  CGIFILVVLLYFVCCKAREKSRRPIYRPVIGEPQPLQVDGGLRNLLFSFTVW
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_010956
• ORF Size: 786 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_010956.1, YP_001974461
• RefSeq ORF: 786
• Locus ID: 6386273
• MW: 29.2 kDa