L4 (NC_010956) Virus Tagged ORF Clone

CAT#: VC100442

Myc-DDK-tagged ORF clone of viral ORF for hexon assembly protein 100K [Human adenovirus D], codon optimized for human cell expression, YP_001974431

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100442 represents NCBI reference of YP_001974431 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGAGCAGCCCAGGAAACAGGAGCAGGAGGAGGACCTGACCACGCATGAACAGCCAAAGATCGAAC
  AAGATCTGGGATTCGAGGAGCCGGCAAGACTCGAACCCCCCCAGGATGAGCAAGAGCACGAGCAGGACGC
  AGGACAGGAAGAGACTGACGCAGGCCTGGAGCATGGGTACCTCGGGGGTGAGGAGGACGTGCTCCTGAAA
  CACCTCCAGAGACAGAGCCTCATCTTGAGAGACGCTCTGGCCGACCGCTCAGAGACTCCTCTGTCTGTAG
  AGGAGCTTTGCCGCGCCTATGAGCTGAACTTGTTCTCACCAAGGGTACCCCCCAAACGCCAACCCAACGG
  GACTTGTGAGCCTAACCCACGCCTCAACTTTTACCCTGTCTTCGCCGTGCCCGAGGCCCTCGCCACATAC
  CATATTTTTTTCAAGAACCAGAAAATACCAGTCTCCTGCCGGGCTAATAGAACCCGGGCGGACGCCCTGC
  TCGCTCTCGGACCAGGCGCCCGGATCCCTGATATCGCAAGTCTGGAAGAAGTTCCGAAGATTTTTGAGGG
  TCTTGGTAGAGACGAGACCCGGGCAGCCAATGCACTCAAAGAGACCGCAGAAGAGGAAGGGCATACATCT
  GCCCTGGTGGAGTTGGAGGGTGATAACGCAAGATTGGCCGTGCTGAAAAGAAGTGTGGAGCTTACCCACT
  TCGCTTACCCTGCCGTTAACCTGCCCCCAAAAGTGATGCGGCACATCATGGATCAGCTCATTATGCCTCA
  TATTGAAGCCCTGGACGAATCACAGGAACAGAGACCAGAAGATGCCCGGCCCGTGGTGTCCGACGAGATG
  CTCGCTCGGTGGCTCGGAACCCGCGAGCCCCAAGCCTTGGAGCAAAGAAGAAAGTTGATGCTGGCCGTCG
  TGCTTGTCACACTTGAGCTGGAGTGTATGCGGAGATTCTTCAGCGACCCAGAGACACTGAGAAAGGTCGA
  GGAGACCTTGCACTATACCTTTCGGCACGGGTTCGTGCGCCAGGCCTGTAAGATCTCCAACGTTGAACTT
  ACAAACCTGGTGTCCTGCCTGGGAATCCTCCACGAGAACCGACTGGGCCAGACAGTGCTTCACAGTACAC
  TGAAAGGTGAGGCAAGGAGAGATTACGTGCGAGACTGCGTGTTCCTGTTCTTGTGCCATACCTGGCAGGC
  CGCTATGGGCGTCTGGCAGCAATGTCTGGAGGACGAAAACTTGAAAGAATTGGACAAACTGTTGGCACGA
  AATCTGAAAAAGCTGTGGACCGGGTTTGACGAAAGAACAGTGGCCAGCGACCTGGCCGAGATTGTCTTTC
  CAGAGCGGCTGCGCCAGACTCTGAAAGGTGGCCTTCCCGACTTCATGTCTCAGAGCATGCTTCAAAACTA
  TCGGACCTTCATCCTCGAACGATCAGGTATGCTCCCCGCAACTTGCAACGCCTTCCCTAGTGATTTCGTA
  CCTCTGAGCTACCGCGAGTGTCCACCTCCCCTTTGGTCCCATTGTTATCTTCTCCAACTGGCTAACTACA
  TCGCGTACCACAGCGACGTGATAGAAGATGTGAGCGGGGAGGGCTTGTTGGAGTGCCATTGCCGATGTAA
  CCTGTGCAGCCCCCACCGATCCTTGGTGTGTAATCCCCAACTGCTGTCCGAGACCCAGGTTATCGGCACT
  TTCGAACTGCAGGGTCCCCAGGAGTCCACCGCTCCACTGAAATTGACCCCAGGTCTGTGGACGTCAGCCT
  ACCTGAGGAAATTTGTGCCCGAGGACTATCACGCTCACGAAATCAAATTTTTTGAGGATCAGAGTCGACC
  TCAGCATGCCGATCTCACAGCATGCGTAATTACTCAGGGCGCCATACTGGCCCAGTTGCATGCTATTCAG
  AAAAGCAGGCAGGAGTTCCTGTTGAAGAAAGGCAGAGGTGTGTACCTTGATCCTCAGACAGGGGAGGTGC
  TTAATACCGGACTCCCCCAACACGCCGAAGAAGAAGCAGGAGCCGCTTCAGGAGGGGATGGACGGAGAAT
  GGGCCAGCCAGGTAGAGGGGGGCGAATGGGGGGAGGAGATAGGGGAGGAAGGATTGGCCGGGGTGGGAGA
  GGAGCCGGCAATAGGGCCGCCAGGAGACGCACTATACGAGCAGGCTCCCCGGGCGGCCACGGATACAACC
  TTCGAAGCTCTGGGCAAGCAAGCTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100442 representing YP_001974431
  Red=Cloning sites Green=Tags
  
  MEEQPRKQEQEEDLTTHEQPKIEQDLGFEEPARLEPPQDEQEHEQDAGQEETDAGLEHGYLGGEEDVLLK
  HLQRQSLILRDALADRSETPLSVEELCRAYELNLFSPRVPPKRQPNGTCEPNPRLNFYPVFAVPEALATY
  HIFFKNQKIPVSCRANRTRADALLALGPGARIPDIASLEEVPKIFEGLGRDETRAANALKETAEEEGHTS
  ALVELEGDNARLAVLKRSVELTHFAYPAVNLPPKVMRHIMDQLIMPHIEALDESQEQRPEDARPVVSDEM
  LARWLGTREPQALEQRRKLMLAVVLVTLELECMRRFFSDPETLRKVEETLHYTFRHGFVRQACKISNVEL
  TNLVSCLGILHENRLGQTVLHSTLKGEARRDYVRDCVFLFLCHTWQAAMGVWQQCLEDENLKELDKLLAR
  NLKKLWTGFDERTVASDLAEIVFPERLRQTLKGGLPDFMSQSMLQNYRTFILERSGMLPATCNAFPSDFV
  PLSYRECPPPLWSHCYLLQLANYIAYHSDVIEDVSGEGLLECHCRCNLCSPHRSLVCNPQLLSETQVIGT
  FELQGPQESTAPLKLTPGLWTSAYLRKFVPEDYHAHEIKFFEDQSRPQHADLTACVITQGAILAQLHAIQ
  KSRQEFLLKKGRGVYLDPQTGEVLNTGLPQHAEEEAGAASGGDGRRMGQPGRGGRMGGGDRGGRIGRGGR
  GAGNRAARRRTIRAGSPGGHGYNLRSSGQASS
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_010956
• ORF Size: 2196 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_010956.1, YP_001974431
• RefSeq ORF: 2196
• Locus ID: 6386277
• MW: 82.2 kDa