L2 (NC_010956) Virus Tagged ORF Clone

CAT#: VC100436

Myc-DDK-tagged ORF clone of viral ORF for core protein V [Human adenovirus D], codon optimized for human cell expression, YP_001974451

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100436 represents NCBI reference of YP_001974451 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTAAACGCAAGATTAAGGAGGAGATGCTGCAGGTGGTGGCCCCCGAGATCTACGGGCCTCCTCAAG
  CAGACCAGAAACCCAGAAAGATCAAGAGAGTCAAAAAGAAGGATGAGGTCGATGAAGGAGCCGTCGAATT
  CGTGAGGGAGTTTGCGCCCAGACGAAGAGTGAATTGGAAAGGCCGCAGAGTGCAGAGAGTGCTGCGACCC
  GGGACAGCCGTGGTCTTCACCCCAGGAGAGAGATCTTCCGTCCGAAGCAAGAGGTCATATGACGAAGTCT
  ATGGCGACGATGATATCCTCGACCAGGCTGCCGAGCGCGCCGGCGAATTCGCATACGGTAAACGGTCCAG
  AGAGGAAGAGCTCATCTCACTGCCTCTGGATGAAAGCAATCCGACTCCAAGCCTGAAGCCAGTCACTCTG
  CAACAGGTCCTGCCACAGGCTGTACTCCTCCCATCCCGAGGTGTAAAGCGAGAGGGTGAAAGCATGTATC
  CAACTATGCAAATCATGGTACCAAAGCGCAGGCGCGTTGAAGATGTCTTGGATACAGTGAAGATGGACGT
  TGAGCCGGAGGTTAAGGTGAGACCCATAAAACAGGTGGCTCCCGGGCTCGGGGTGCAGACTGTCGACATC
  CAGATCCCTACTGACATGGACGTGGACAAGAAACCCTCCACAAGTATTGAGGTCCAGACTGACCCCTGGC
  TGCCTGCGAGCACTGCTACCGTATCAACGTCAACCGCCGCTACTGCAACAGAACCCCCTCGACGCCGGCG
  GTGGGGCGCCGCTTCACGGCTGATGCCGAACTATGTCGTCCACCCAAGTATCATCCCTACACCCGGCTAC
  CGGGGCACCCGGTATTATGCTTCACGCAGACGCCCGGCCTCTAAACGAAGACGGAGAACAGCTACTCGGC
  GCCGCTTGGCCCCAGCCCGGGTGAGGCGGGTTACAACTAGGCGAGGTAGATCCCTCGTGCTTCCAACAGT
  AAGATATCATCCGAGTATTCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100436 representing YP_001974451
  Red=Cloning sites Green=Tags
  
  MSKRKIKEEMLQVVAPEIYGPPQADQKPRKIKRVKKKDEVDEGAVEFVREFAPRRRVNWKGRRVQRVLRP
  GTAVVFTPGERSSVRSKRSYDEVYGDDDILDQAAERAGEFAYGKRSREEELISLPLDESNPTPSLKPVTL
  QQVLPQAVLLPSRGVKREGESMYPTMQIMVPKRRRVEDVLDTVKMDVEPEVKVRPIKQVAPGLGVQTVDI
  QIPTDMDVDKKPSTSIEVQTDPWLPASTATVSTSTAATATEPPRRRRWGAASRLMPNYVVHPSIIPTPGY
  RGTRYYASRRRPASKRRRRTATRRRLAPARVRRVTTRRGRSLVLPTVRYHPSIL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_010956
• ORF Size: 1002 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_010956.1, YP_001974451
• RefSeq ORF: 1002
• Locus ID: 6386285
• MW: 38.0 kDa