E3 (NC_010956) Virus Tagged ORF Clone

CAT#: VC100447

Myc-DDK-tagged ORF clone of viral ORF for membrane glycoprotein E3 CR1-alpha [Human adenovirus D], codon optimized for human cell expression, YP_001974458

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100447 represents NCBI reference of YP_001974458 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCATTTTTGCTGTCCTGTTCGTGGTATCACTGATTAAAGCTAAACTGTTGCAATACTCTGGCCCAT
  GCCGGCACCAGTTGAATGAGACCGTTTACCTGACCAACCAAACTGAGGTTAAGCTCACTTGCCGCCCACA
  CAAGACCTACATAATCTGGTTTTTCGAGAACACTTCATTTGCCGTTAGCAACACACATTGCAATGACGGC
  GTGGAGCTTCCGAACAATCTGAGCTCAGGCCTTTCCTATGACACTCATAGAGCGAAACTCGTGCTGTATA
  ACCCCTTTGTCGAGGGGACCTATCACTGTCAGTCTGGGCCGTGCTTCCATTCCTTTACACTGGTGAACGT
  GACAGGATCTTCAACCGCCGCCCCCGAGCCCAATCTCCCTAGTGACACAATCAAACCAAGATTTGGCGGA
  GAGCTGAGACTGCCACCTAGCGAAGAAGGGGTTTCTCCATATGAAGTGGTCGGGTACCTGATCCTCGGAG
  TCGTCCTTGGTGGCTGTATCGCGGTCCTGGCCCAGTTGCCCTGTTGGGTTGAGATTAAAATTTTTATTTG
  TTGGGTGAGACACTGCGGAGAGGAGCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100447 representing YP_001974458
  Red=Cloning sites Green=Tags
  
  MRIFAVLFVVSLIKAKLLQYSGPCRHQLNETVYLTNQTEVKLTCRPHKTYIIWFFENTSFAVSNTHCNDG
  VELPNNLSSGLSYDTHRAKLVLYNPFVEGTYHCQSGPCFHSFTLVNVTGSSTAAPEPNLPSDTIKPRFGG
  ELRLPPSEEGVSPYEVVGYLILGVVLGGCIAVLAQLPCWVEIKIFICWVRHCGEEL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_010956
• ORF Size: 588 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_010956.1, YP_001974458
• RefSeq ORF: 588
• Locus ID: 6386273
• MW: 21.8 kDa