E2B (NC_010956) Virus Tagged ORF Clone

CAT#: VC100429

Myc-DDK-tagged ORF clone of viral ORF for DNA polymerase [Human adenovirus D], codon optimized for human cell expression, YP_001974426

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E2B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100429 represents NCBI reference of YP_001974426 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCACTGGTCCAGTCTCACGGCGCCAGAGGTCTTCACGCAGAAGCCGCTGACCCAGGCTGTCAACCTC
  CACGCAGGCGGGCGAGACAGCGATCTCAAGGAGCTGCTCCTGGACCCGCTAGAGCACCACGGAGAAGAGC
  CTCTGCAGCACCAGCTAGAGGAGCTAGGACTGCCGCCGCAGCGGGAAGCACTCCAGCCACACCCCTGCTG
  AAAGCCCACAGAGGCACTGTGGTGGCCCCTCGGTCTTACGGTCTCATGCAGTGCGTCGATACCACTACAA
  ACAGCCCTGTTGAAATTAAATACCACTTGCATCTGAAGCATGCATTGACTAGATTGTACGAAGTCAATCT
  TCGAACACTGCCACCCGACCTTGATCTGAGAGATACCATGGACAGCTCACAGCTGAGGGCCTTGGTTTTT
  GCGCTCAGACCACGCCGCGCAGAGATCTGGACATGGCTGCCACGAGGACTGGTATCTTTGAGCGTACTGG
  AAGAACCTCAGGGTGAGTCACATGCTGGCGAACATGAGTCTCACCAGCCAGGGCCTCCCCTGCTTAAGTT
  TCTCCTGAAAGGTCGGGCGGTGTACCTGGTTGACGAGGTCCAGCCAGTACAACGGTGCGAATATTGTGGC
  CGCTTCTACAAGCATCAGCACGAGTGCAGCGTGAGAAGGCGGGACTTTTACTTCCATCACATCAACTCCC
  ATAGCTCAAATTGGTGGCAAGAGATCCAGTTTTTCCCCATTGGCAGTCACCCTCGAACCGAGCGACTCTT
  CGTGACATACGACGTCGAAACATACACGTGGATGGGAAGTTTCGGAAAGCAGCTGGTTCCCTTTATGCTG
  GTGATGAAGTTCAGCGGGGAGCCAGAGCTGGTGGCTCTGGCTAGGGATCTCGCGGTAAGACTCAGATGGG
  ATAGGTGGGAGAGAGATCCACTGACCTTTTACTGTGTCACTCCAGAAAAGATGGCCGTGGGCCAGCAGTT
  CCGACTGTTTAGGGATGAGCTCCAGACATTGATGGCGCGCGAGCTGTGGGCCTCATTCATGCAGGCCAAT
  CCCCACTTGCAGGAATGGGCCTTGGAACAGCATGGTCTTCAGTGCCCGGAGGATCTGACGTATGAGGAGT
  TGAAAAAGCTGCCTCATATAAAGGGCAGACCCCGGTTCATGGAATTGTATATCGTGGGACATAATATAAA
  CGGGTTCGACGAAATCGTGCTGGCTGCGCAGGTGATCAATAACCGGGCTTCTGTGCCAGGACCTTTTCGG
  ATCACCCGCAATTTCATGCCCAGGGCCGGAAAGATTCTGTTTAATGATGTGACCTTCGCCCTGCCGAACC
  CTCTGTCCAAAAAGAGAACCGATTTCGAACTGTGGGAGCATGGAGGCTGTGATGATTCTGACTTCAAGTA
  TCAGTTCCTTAAAGTCATGGTGAGAGACACCTTTGCACTGACCCATACCTCCCTCCGGAAAGCAGCCCAG
  GCCTACGCACTGCCCGTCGAAAAGGGATGTTGTCCTTACAAGGCAGTGAACCATTTCTACATGTTGGGAA
  GCTATCGAGCCGATGATAGGGGCTTTCCACTGAGAGAGTATTGGAAGGATGATGAAGAGTATGCCCTCAA
  CAGAGAACTGTGGGAAAAAAAAGGGGAAGCAGGTTATGACATAATAAGAGAGACACTGGACTACTGTGCC
  ATGGATGTGCTGGTCACAGCCGAGCTTGTGGCGAAGCTTCAGGACTCCTACGCTCATTTCATCAGGGACA
  GCGTCCGCCTGCCTCACGCTCACTTCAACATCTTCCAGCGGCCTACCATTAGCAGCAATAGCCATGCCAT
  CTTTCGACAGATCGTCTTCCGAGCTGAGCAACCGCAAAGAACCAACCTCGGCCCAGCTTTCCTGGCGCCT
  TCCCACGAGTTGTACGATTATGTGCGCGCAAGCATTCGCGGCGGCCGCTGCTACCCTACATACATCGGGA
  TTCTGTCCGAGCCAATCTACGTTTACGACATATGCGGCATGTACGCCTCCGCCCTCACTCACCCAATGCC
  CTGGGGACCCCCCCTCAATCCTTACGAGCGGGCACTGGCTGCACGCGAATGGCAGATGGCTCTCGACGAC
  GCTTCTTCCAAAATTGACTACTTTGATAAAGAGCTGTGTCCCGGGATTTTTACCATTGACGCAGATCCAC
  CTGATGAACACTTGCTGGATGTACTCCCGCCGTTCTGTAGTCGCAAAGGCGGGCGCCTGTGCTGGACTAA
  TGAGCCTCTCCGAGGCGAGGTGGCTACCTCTGTAGACCTTGTGACCCTCCATAACAGGGGGTGGCGCGTG
  CGGATAGTGCCTGATGAACGGACAACCGTTTTCCCGGAATGGAAGTGTGTGGCTAGAGAGTATGTACAGC
  TGAATATCGCTGCCAAGGAGCGAGCCGATCGGGATAAAAACCAGACCATGCGGTCAATTGCTAAGTTGTT
  GTCCAACGCTCTCTACGGCAGCTTTGCCACAAAACTGGACAACAAGAAAATCGTCTTCTCTGACCAAATG
  GATGAGTCTCTGTTGAAGTCAATCGCCGCTGGTCAGGCGAACATAAAAAGTAGTTCATTTCTCGAGACCG
  ACAATCTGTCTGCTGAGGTGATGCCCGCACTGGAGCGCGAGTATTTGCCCCAGCAGCTGGCACTTGTCGA
  TAGTGACGCCGAGGAGTCTGAAGACGAACATAGACCTGCTCCATTCTACACACCACCTTCAGGCACTCCC
  GGCCATGTGGCATATACCTATAAGCCTATCACTTTTCTGGACGCCGAGGAAGGCGATATGTGTCTGCACA
  CGGTCGAAAAAGTGGATCCCCTGGTGGATAACGACCGATATCCTAGCCATGTCGCGTCTTTCGTGCTGGC
  CTGGACGCGAGCCTTCGTGAGTGAGTGGAGCGAGTTTCTGTACGAAGAGGATAGGGGGACACCTCTCCAG
  GATAGACCGATTAAGAGTGTGTATGGTGATACAGATTCACTGTTCGTGACCGAGAGAGGCCACCGCCTCA
  TGGAGACCAGGGGGAAGAAGCGCATCAAGAAAAACGGAGGAAAATTGGTATTTGACCCAGAGCAACCAGA
  ACTCACCTGGCTCGTCGAGTGCGAAACTGTATGCGCGCACTGTGGGGCCGACGCTTTTGCACCAGAAAGC
  GTATTTCTGGCGCCTAAGCTGTACGCCCTGCAATCATTGCTGTGCCCAGCTTGTGGACGATCCAGCAAGG
  GAAAGCTGAGAGCAAAAGGGCACGCTGCTGAGGCTCTGAACTATGAGCTGATGGTTAACTGTTATCTGGC
  TGACTCCCAGGGCGAGGATAGGGCTCGGTTTTCCACCAGTCGCATGTCACTGAAGCGAACTCTCGCGAGC
  GCGCAGCCCGGAGCTCATCCCTTCACCGTAACAGAGACCACACTGACCAGAACTCTGAGGCCATGGAAGG
  ACATGACACTGGCCGCGCTGGACGCCCACAGACTGGTGCCATACAGCCGCAGTAGGCCTAACCCACGGAA
  CGAGGAGGTGTGTTGGATCGAGATGCCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100429 representing YP_001974426
  Red=Cloning sites Green=Tags
  
  MALVQSHGARGLHAEAADPGCQPPRRRARQRSQGAAPGPARAPRRRASAAPARGARTAAAAGSTPATPLL
  KAHRGTVVAPRSYGLMQCVDTTTNSPVEIKYHLHLKHALTRLYEVNLRTLPPDLDLRDTMDSSQLRALVF
  ALRPRRAEIWTWLPRGLVSLSVLEEPQGESHAGEHESHQPGPPLLKFLLKGRAVYLVDEVQPVQRCEYCG
  RFYKHQHECSVRRRDFYFHHINSHSSNWWQEIQFFPIGSHPRTERLFVTYDVETYTWMGSFGKQLVPFML
  VMKFSGEPELVALARDLAVRLRWDRWERDPLTFYCVTPEKMAVGQQFRLFRDELQTLMARELWASFMQAN
  PHLQEWALEQHGLQCPEDLTYEELKKLPHIKGRPRFMELYIVGHNINGFDEIVLAAQVINNRASVPGPFR
  ITRNFMPRAGKILFNDVTFALPNPLSKKRTDFELWEHGGCDDSDFKYQFLKVMVRDTFALTHTSLRKAAQ
  AYALPVEKGCCPYKAVNHFYMLGSYRADDRGFPLREYWKDDEEYALNRELWEKKGEAGYDIIRETLDYCA
  MDVLVTAELVAKLQDSYAHFIRDSVRLPHAHFNIFQRPTISSNSHAIFRQIVFRAEQPQRTNLGPAFLAP
  SHELYDYVRASIRGGRCYPTYIGILSEPIYVYDICGMYASALTHPMPWGPPLNPYERALAAREWQMALDD
  ASSKIDYFDKELCPGIFTIDADPPDEHLLDVLPPFCSRKGGRLCWTNEPLRGEVATSVDLVTLHNRGWRV
  RIVPDERTTVFPEWKCVAREYVQLNIAAKERADRDKNQTMRSIAKLLSNALYGSFATKLDNKKIVFSDQM
  DESLLKSIAAGQANIKSSSFLETDNLSAEVMPALEREYLPQQLALVDSDAEESEDEHRPAPFYTPPSGTP
  GHVAYTYKPITFLDAEEGDMCLHTVEKVDPLVDNDRYPSHVASFVLAWTRAFVSEWSEFLYEEDRGTPLQ
  DRPIKSVYGDTDSLFVTERGHRLMETRGKKRIKKNGGKLVFDPEQPELTWLVECETVCAHCGADAFAPES
  VFLAPKLYALQSLLCPACGRSSKGKLRAKGHAAEALNYELMVNCYLADSQGEDRARFSTSRMSLKRTLAS
  AQPGAHPFTVTETTLTRTLRPWKDMTLAALDAHRLVPYSRSRPNPRNEEVCWIEMP
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_010956
• ORF Size: 3528 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_010956.1, YP_001974426
• RefSeq ORF: 3528
• Locus ID: 6386271
• MW: 134.0 kDa