L3 (NC_010956) Virus Tagged ORF Clone

CAT#: VC100439

Myc-DDK-tagged ORF clone of viral ORF for hexon [Human adenovirus D], codon optimized for human cell expression, YP_001974453

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100439 represents NCBI reference of YP_001974453 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTACCCCGTCTATGATGCCCCAGTGGGCATATATGCATATTGCCGGCCAGGATGCTTCTGAATACC
  TGTCTCCCGGTCTGGTTCAATTCGCACGCGCTACCGACACGTACTTCTCTTTGGGCAATAAATTTAGGAA
  TCCAACAGTAGCACCAACACACGACGTGACCACCGATCGGAGCCAGCGCTTGACACTGCGCTTCGTGCCC
  GTCGACCGCGAGGACACAACGTACTCCTACAAAGCCCGGTTCACTCTGGCTGTGGGCGACAATAGAGTCC
  TGGATATGGCCTCCACTTACTTTGATATTCGAGGAGTGCTGGATCGGGGACCTTCCTTCAAACCTTACTC
  CGGGACCGCCTACAATAGCCTGGCTCCTAAAGGCGCCCCAAATAGTTCACAGTGGTTGGCAAAGGATACC
  AACGCCGGAGACCAGGCCCTTAAGACCCACACTCACGGAGTGGCAGCCATGGGAGGCACTGACATTACCG
  CCAAGGGACTCCAGATCGGGGTTGATACAACAGAAAACAAAAATGAGCCTATTTATGCAAACGAAATTTA
  TCAGCCGGAGCCACAGGTTGGTGAAGAAAATTTGCAGGATGTGGAGAACTTTTACGGCGGGCGCGCTCTG
  AAGAAGGAGACCAAGATGAAACCTTGCTACGGCAGCTTTGCCAGGCCAACAAACGAAAAGGGAGGACAAG
  CTAAATTCCTCACCGACGGCGATGGGCAGCTGACCAAAAACCATGACATTACAATGAATTTCTTCGACAC
  CCCAGGGGGGACTGTGGGGCAGGACACAGAGCTGGAAGCTGACATAGTGATGTACGCCGAAAATGTCCAC
  CTGGAAACCCCAGATACTCATGTTGTGTACAAACCCGGAACATCCGATGAGTCTTCAGAGGCCAACCTTG
  TGCAGCAATCCATGCCAAATAGGCCAAACTATATTGGATTTAGGGACAACTTCGTAGGGCTCATGTACTA
  CAACTCCACCGGAAACATGGGGGTCCTTGCAGGTCAGGCCTCACAGCTTAACGCAGTCGTAGACCTGCAG
  GACCGCAACACAGAGCTGTCATACCAACTCCTTCTGGATTCTCTGGGCGACAGAACCAGATACTTTTCCA
  TGTGGAACAGCGCCGTGGATTCATACGATCCAGACGTGAGGATTATCGAAAACCACGGCGTTGAGGACGA
  GTTGCCGAATTACTGCTTCCCCCTGGATGGTGCAGGTACAAACGCTACCTACCAGGGCGTGAAAGTCAAA
  AACGGCCAAGACGGTGATGTAAATGCGGATTGGGAGAAGGATCCGAACCTGGCGTCACGCAACCAGATTT
  GTAAAGGCAACATATTTGCTATGGAGATTAATCTGCAAGCGAACCTTTGGAAAAGCTTTTTGTACTCTAA
  TGTTGCTTTGTATTTGCCCGACAGCTACAAATACACCCCTGCCAACGTGACACTGCCCGCCAATACAAAC
  ACGTATGAGTACATGAACGGAAGGGTCGTGGCTCCTTCCCTCGTAGATGCTTATATTAACATCGGCGCAC
  GGTGGTCCCTTGATCCCATGGACAACGTTAACCCCTTCAACCACCACCGCAACGCTGGGCTTAGATATAG
  ATCCATGTTGCTCGGGAATGGGCGGTACGTGCCGTTTCATATTCAAGTCCCCCAGAAATTCTTTGCGATA
  AAAAACCTTCTGCTGCTTCCTGGGAGTTATACTTATGAATGGAACTTTAGGAAAGACGTCAATATGATCC
  TGCAATCCAGTCTGGGGAACGATCTGCGAGTTGACGGAGCTTCTGTACGGTTTGACTCAGTGAACCTGTA
  CGCCACATTCTTTCCGATGGCACACAACACTGCTTCCACACTGGAAGCCATGCTGCGAAACGACACCAAT
  GACCAATCCTTCAACGATTACCTTTCAGCAGCAAATATGCTGTACCCGATCCCTGCCAAGGCCACGAACG
  TACCAATATCTATACCTAGCAGAAACTGGGCGGCATTTAGAGGCTGGAGCTTCACCCGGCTGAAGACCAA
  GGAAACTCCCTCCTTGGGGAGCGGGTTTGATCCCTACTTTGTGTATTCCGGGTCCATTCCGTACCTGGAT
  GGGACATTCTACTTGAACCACACGTTCAAGAAGGTATCAATCATGTTCGACTCTAGTGTCAGCTGGCCAG
  GCAACGACCGGCTCCTTACGCCAAACGAATTCGAGATCAAGCGCTCTGTCGACGGGGAAGGATACAACGT
  GGCGCAGTGTAATATGACTAAAGACTGGTTTCTCGTGCAGATGCTCTCTCATTACAACATTGGTTACCAG
  GGCTTTCATGTGCCGGAGGGCTATAAGGACCGAATGTATTCTTTTTTTCGCAACTTTCAGCCGATGAGTA
  GACAGGTCGTTGATGAAATTAATTATAAAGACTATAAAGCTGTCACCTTGCCCTTCCAACACAACAACAG
  TGGATTCACCGGGTACCTCGCACCTACCATGAGACAGGGACAGCCCTATCCCGCTAATTTTCCCTACCCG
  CTGATTGGTCAGACCGCGGTGCCCTCAGTGACCCAAAAAAAATTTCTGTGCGACCGCGTGATGTGGCGGA
  TCCCATTTTCCTCTAATTTCATGTCTATGGGAGCACTCACAGATTTGGGACAAAATATGCTTTACGCCAA
  TAGCGCTCACGCCCTGGATATGACATTTGAGGTCGATCCTATGGATGAACCCACACTGCTCTACTTGTTG
  TTCGAAGTGTTCGACGTGGTCCGGGTGCATCAGCCACATAGGGGCGTGATCGAAGCTGTCTACTTGAGAA
  CTCCCTTTAGCGCCGGCAATGCCACTACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100439 representing YP_001974453
  Red=Cloning sites Green=Tags
  
  MATPSMMPQWAYMHIAGQDASEYLSPGLVQFARATDTYFSLGNKFRNPTVAPTHDVTTDRSQRLTLRFVP
  VDREDTTYSYKARFTLAVGDNRVLDMASTYFDIRGVLDRGPSFKPYSGTAYNSLAPKGAPNSSQWLAKDT
  NAGDQALKTHTHGVAAMGGTDITAKGLQIGVDTTENKNEPIYANEIYQPEPQVGEENLQDVENFYGGRAL
  KKETKMKPCYGSFARPTNEKGGQAKFLTDGDGQLTKNHDITMNFFDTPGGTVGQDTELEADIVMYAENVH
  LETPDTHVVYKPGTSDESSEANLVQQSMPNRPNYIGFRDNFVGLMYYNSTGNMGVLAGQASQLNAVVDLQ
  DRNTELSYQLLLDSLGDRTRYFSMWNSAVDSYDPDVRIIENHGVEDELPNYCFPLDGAGTNATYQGVKVK
  NGQDGDVNADWEKDPNLASRNQICKGNIFAMEINLQANLWKSFLYSNVALYLPDSYKYTPANVTLPANTN
  TYEYMNGRVVAPSLVDAYINIGARWSLDPMDNVNPFNHHRNAGLRYRSMLLGNGRYVPFHIQVPQKFFAI
  KNLLLLPGSYTYEWNFRKDVNMILQSSLGNDLRVDGASVRFDSVNLYATFFPMAHNTASTLEAMLRNDTN
  DQSFNDYLSAANMLYPIPAKATNVPISIPSRNWAAFRGWSFTRLKTKETPSLGSGFDPYFVYSGSIPYLD
  GTFYLNHTFKKVSIMFDSSVSWPGNDRLLTPNEFEIKRSVDGEGYNVAQCNMTKDWFLVQMLSHYNIGYQ
  GFHVPEGYKDRMYSFFRNFQPMSRQVVDEINYKDYKAVTLPFQHNNSGFTGYLAPTMRQGQPYPANFPYP
  LIGQTAVPSVTQKKFLCDRVMWRIPFSSNFMSMGALTDLGQNMLYANSAHALDMTFEVDPMDEPTLLYLL
  FEVFDVVRVHQPHRGVIEAVYLRTPFSAGNATT
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_010956
• ORF Size: 2829 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_010956.1, YP_001974453
• RefSeq ORF: 2829
• Locus ID: 6386283
• MW: 106.1 kDa