E2A (NC_010956) Virus Tagged ORF Clone

CAT#: VC100441

Myc-DDK-tagged ORF clone of viral ORF for single-stranded DNA-binding protein [Human adenovirus D], codon optimized for human cell expression, YP_001974427

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E2A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100441 represents NCBI reference of YP_001974427 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGGTGGCTCACAGGACGTCCGCAGGTTTATGGAGCGGGAGGCAACCCCTCCTCGAGGCCACGGAT
  CAGCTCGCTATCCTCCAGAACAGGAGCGCAGTCCCAGTCCGCCCCCTCCACTCCCCACTAAACGAAGGAA
  GTACCAGCGGGTCGGAAGTGGCTCCAGCGAAGAGGATGTGGTCCCGGTGGACTCTCCACCTAAAAAAAAA
  CAGGCTAGAAAAACTAAGCACGTCACTAAAGTCGATCCTGACGAAGAGATGCCCCAGGAAGACGCTGTGA
  TTGTGGGGGTCGGGTTCAGTCAACCCCCGGTCCTCCTGAAGGAAGGTAAGGACGGTAAGAGGATAGTTGA
  ACCTGCCACCCCTGGAATCCTCAACGTGCGCAATCCTCTGAGTCTGCCCCTTGTGAGCTCATGGGAAAAG
  GGAATGGATACCATGAACGTGCTTATGGAGCGCTATCGCGTCGATTCCGGCCTCAGAGACGCATATAAAT
  TGATGCCAGAACAGACCGAAATCTTTCAAAAGATGTGCCAAACATGGATGAACGAGGAAGCTAGAGGGCT
  GCAATTGACTTTTTCCACCTCCAAAAGTTTTTCCACCGTCATGGGGCGCCTGCTGCAGGGCTACATCTTT
  TCCCACTCTGGGATTGCCCATAAGAACTGGGAGTGTACAGGGTGCGCTCTCTGGGACCATGGATGTACTG
  AGGTTGAAGGCCAGTTGAAATGTCTTCATGGAACGGTTATGATCCATAAAGATCATGTTGTGGAGATGGA
  CGTGACTTCAGAGAACGGACAAAGAGCCCTCAAAGAGCAGCCAAGTAAAGCCAAAGTAACTCAGAATCGA
  TGGGGGCGGAGCGTGGTACAGCTCACTTCCCATGACGCCCGATGTTGTGTGCATGACGCTGGCTGTGGGA
  ACAACCAGTTTAGCGGCAAGTCCTGCGGCTTGTTCTTTTCTGAAGGGGCCAAGGCTCAGCAGGCCTTCAA
  GCAGATCTCTGCATTTGTTAAGGCCCTGTACCCAAATATGCAGAGAGGGGCAGGGATGATGCTCATGCCA
  GTGCATTGCGAATGTAATCACAAACCCCAAAGCGTGCCCTTCCTCGGACGCCAACTCTGCAAGATGACTC
  CCTTCGGTCTTAGCAACGCAGAAGACTTGGATAAAGATCAGATCAGCGACAAGAGCGTGCTGGCCTCTGT
  TAAATACCCATCCCTGATGGTGTTCCAATGCTGTAACCCAGTCTACCGAAATAGCCGGGCGCAGTCAACC
  GGTCCTAACTGTGACTTCAAGATATCCGCCCCTGATATCCTGGGCGCACTTCAGATGTCTAGGCGGATGT
  GGTCAGAGACCTTTCCCGAAACCCCCGTCCCAAAGCTTGTGATCCCAGAGTTTAAGTGGCAGCCGAAATT
  TCAGTACCGGAATGTGGCCCTCCCATCCGCTGCACATAACGATGAAAGGGAGAATCCTTTTGACTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100441 representing YP_001974427
  Red=Cloning sites Green=Tags
  
  MAGGSQDVRRFMEREATPPRGHGSARYPPEQERSPSPPPPLPTKRRKYQRVGSGSSEEDVVPVDSPPKKK
  QARKTKHVTKVDPDEEMPQEDAVIVGVGFSQPPVLLKEGKDGKRIVEPATPGILNVRNPLSLPLVSSWEK
  GMDTMNVLMERYRVDSGLRDAYKLMPEQTEIFQKMCQTWMNEEARGLQLTFSTSKSFSTVMGRLLQGYIF
  SHSGIAHKNWECTGCALWDHGCTEVEGQLKCLHGTVMIHKDHVVEMDVTSENGQRALKEQPSKAKVTQNR
  WGRSVVQLTSHDARCCVHDAGCGNNQFSGKSCGLFFSEGAKAQQAFKQISAFVKALYPNMQRGAGMMLMP
  VHCECNHKPQSVPFLGRQLCKMTPFGLSNAEDLDKDQISDKSVLASVKYPSLMVFQCCNPVYRNSRAQST
  GPNCDFKISAPDILGALQMSRRMWSETFPETPVPKLVIPEFKWQPKFQYRNVALPSAAHNDERENPFDF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_010956
• ORF Size: 1467 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_010956.1, YP_001974427
• RefSeq ORF: 1467
• Locus ID: 6386281
• MW: 54.8 kDa