E4 (NC_010956) Virus Tagged ORF Clone

CAT#: VC100460

Myc-DDK-tagged ORF clone of viral ORF for control protein E4orf2 [Human adenovirus D], codon optimized for human cell expression, YP_001974473

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100460 represents NCBI reference of YP_001974473 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCAGCGCAGGGGGGTGTCCTACCATATCGTGGTTCCCGGAGTGCTCGTTACATACCTCGAAGATT
  TCAGTATTACACAGATGATCAAGGAGAAGTTGCCCCGCTTTATCACGCACATCCTTGAGGGAATTACAGG
  GGATACCAAGCGCGCTTACTCCTCTATGCAGTTTCTGGGAGCGTCATTTGGTGCTCTGAAGTTTTCTCTC
  ACCCTGGCATCCCCTACACTTAGCCCCGGGAGCGAACTTTCCGCTGTGGTGGCCCAGGACCTGAGTGACT
  TCCTCCAGTTGACGTTGAGGCGAGAGCTGAGAGCTGAGGGGAGAACTTTGCTGAACCTGGTGGTGCTCAA
  CACTCTTCAGGTCGTGGAGCAGCAGGATCTCTTTCTCCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100460 representing YP_001974473
  Red=Cloning sites Green=Tags
  
  MLQRRGVSYHIVVPGVLVTYLEDFSITQMIKEKLPRFITHILEGITGDTKRAYSSMQFLGASFGALKFSL
  TLASPTLSPGSELSAVVAQDLSDFLQLTLRRELRAEGRTLLNLVVLNTLQVVEQQDLFLL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_010956
• ORF Size: 390 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_010956.1, YP_001974473
• RefSeq ORF: 390
• Locus ID: 6386282
• MW: 14.5 kDa