L3 (NC_010956) Virus Tagged ORF Clone

CAT#: VC100438

Myc-DDK-tagged ORF clone of viral ORF for capsid protein precursor pVI [Human adenovirus D], codon optimized for human cell expression, YP_001974429

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100438 represents NCBI reference of YP_001974429 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGACATAAACTTTGCTAGTCTCGCTCCCCGCCATGGCACCAGACCTTTTATGGGGACTTGGAACG
  AGATCGGGACGTCACAGCTGAACGGTGGCGCTTTCAACTGGAGCAGCGTGTGGTCCGGGCTCAAAAATTT
  TGGCTCCACTCTGCGGACATACGGGAATAAGGCCTGGAATAGTTCAACCGGCCAACTCCTCAGGGAAAAA
  TTGAAGGACCAGAACTTTCAGCAAAAGGTTGTGGACGGCTTGGCAAGCGGGATCAATGGTGTGGTAGATA
  TTGCAAACCAGGCAGTGCAGCGCGAAATCAATTCTAGGTTGGACCCTCGCCCCCCCACAGTGGTGGAAAT
  GGAAGACGCTACTCTGCCCCCACCTAAGGGGGAAAAACGGCCAAGGCCAGATGCTGAAGAAACCATCCTG
  CAGGTGGATGAGCCCCCGAGCTATGAGGAGGCGGTGAAAGCTGGGATGCCAACAACCCGGATCATTGCCC
  CCCTCGCCACTGGCGTGATGAAACCCGCCACCCTGGATCTGCCTCCTCCCCCTGCTCCAGCACCTCCCAA
  GGCCGCACCTGCAGTTGTTCAGGCTCCACCAGTGGCCACTGCCGTAAGGCGGGTTCCTGCCAGGAGACAA
  GCTCAGAACTGGCAGTCAACCCTGCATTCTATAGTCGGCTTGGGGGTTAAGTCTCTGAAGAGGCGCAGGT
  GCTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100438 representing YP_001974429
  Red=Cloning sites Green=Tags
  
  MEDINFASLAPRHGTRPFMGTWNEIGTSQLNGGAFNWSSVWSGLKNFGSTLRTYGNKAWNSSTGQLLREK
  LKDQNFQQKVVDGLASGINGVVDIANQAVQREINSRLDPRPPTVVEMEDATLPPPKGEKRPRPDAEETIL
  QVDEPPSYEEAVKAGMPTTRIIAPLATGVMKPATLDLPPPPAPAPPKAAPAVVQAPPVATAVRRVPARRQ
  AQNWQSTLHSIVGLGVKSLKRRRCY
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_010956
• ORF Size: 705 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_010956.1, YP_001974429
• RefSeq ORF: 705
• Locus ID: 6386283
• MW: 25.6 kDa